Effect of storage for 24 h at 18°C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation

被引：6
作者：
Am-in, N. [1 ]
Kirkwood, R. N. [2 ]
Techakumphu, M. [1 ]
Tantasuparuk, W. [1 ]
机构：
[1] Chulalongkorn Univ, Dept Obstet Gynaecol & Reprod, Fac Vet Sci, Bangkok 10330, Thailand
[2] Michigan State Univ, Dept Large Anim Clin Sci, E Lansing, MI 48824 USA
来源：
CANADIAN JOURNAL OF ANIMAL SCIENCE | 2010年 / 90卷 / 03期
关键词：
Boars; sperm motility; sperm quality; lipid peroxidation; BOAR SEMEN; SPERMATOZOA;
D O I：
10.4141/CJAS09122
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Am-in, N., Kirkwood, R. N., Techakumphu, M. and Tantasuparuk, W. 2010. Effect of storage for 24 h at 18 degrees C on sperm quality and a comparison of two assays for sperm membrane lipid peroxidation. Can. J. Anim. Sci. 90: 389-392. Boars having normal (71.1 +/- 1.2%; n = 10) or low (35.12 +/- 3.9%; n = 10) sperm motility 24 h after collection were used, and semen was evaluated following storage in Beltsville Thawing Solution (BTS) for 24 h at 18 degrees C. Sperm lipids were extracted and lipid peroxidation quantified. No differences were evident in fresh semen, but after 24 h, sperm motility, viability and membrane permeability in the low motility group were lower (P <0.001) compared with the normal motility group. Sperm membrane lipid peroxidation was greater (P <0.001) in the low motility group. A factor influencing sperm storability is membrane lipid peroxidation, which can be accurately assayed using a commercial kit.
引用
收藏
页码：389 / 392
页数：4
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