Integrative Expression System for Delivery of Antibody Fragments by Lactobacilli

被引：39

作者：

Cruz Martin, M. ^{[2
]}

Pant, Neha ^{[1
]}

Ladero, Victor ^{[2
]}

Gunaydin, Gokce ^{[1
]}

Andersen, Kasper Krogh ^{[1
]}

Alvarez, Beatriz ^{[1
]}

Martinez, Noelia ^{[2
]}

Alvarez, Miguel A. ^{[2
]}

Hammarstrom, Lennart ^{[1
]}

Marcotte, Harold ^{[1
]}

机构：

[1] Karolinska Inst, Karolinska Univ Hosp Huddinge, Div Clin Immunol, Dept Lab Med, SE-14186 Stockholm, Sweden

[2] CSIC, IPLA, Villaviciosa 3300, Asturias, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 06期

关键词：

LACTIC-ACID BACTERIA; AGGREGATION-PROMOTING FACTOR; SITE-SPECIFIC RECOMBINATION; CHAIN ANTIBODY; SURFACE; IDENTIFICATION; CONSTRUCTION; VECTORS; GASSERI; PROTEIN;

D O I：

10.1128/AEM.02690-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A series of expression cassettes which mediate secretion or surface display of antibody fragments was stably integrated in the chromosome of Lactobacillus paracasei. L. paracasei producing surface-anchored variable domain of llama heavy chain (VHH) (ARP1) directed against rotavirus showed efficient binding to rotavirus and protection in the mouse model of rotavirus infection.

引用

页码：2174 / 2179

页数：6

共 19 条

[1] The site-specific recombination system of the Lactobacillus species bacteriophage A2 integrates in Gram-positive and Gram-negative bacteria [J].

Alvarez, MA ;

Herrero, M ;

Suárez, JE .

VIROLOGY, 1998, 250 (01) :185-193

[2] Surface display of foreign epitopes on the Lactobacillus brevis S-layer [J].

Åvall-Jääskeläinen, S ;

Kylä-Nikkilä, K ;

Kahala, M ;

Miikkulainen-Lahti, T ;

Palva, A .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) :5943-5951

[3] Lactobacilli-expressed single-chain variable fragment (scFv) specific for intercellular adhesion molecule 1 (ICAM-1) blocks cell-associated HIV-1 transmission across a cervical epithelial monolayer [J].

Chancey, Caren J. ;

Khanna, Kristen V. ;

Seegers, Jos F. M. L. ;

Zhang, Guang Wen ;

Hildreth, James ;

Langan, Abigail ;

Markham, Richard B. .

JOURNAL OF IMMUNOLOGY, 2006, 176 (09) :5627-5636

[4] Potential and opportunities for use of recombinant lactic acid bacteria in human health [J].

Hanniffy, S ;

Wiedermann, U ;

Repa, A ;

Mercenier, A ;

Daniel, C ;

Fioramonti, J ;

Tlaskolova, H ;

Kozakova, H ;

Israelsen, H ;

Madsen, S ;

Vrang, A ;

Hols, P ;

Delcour, J ;

Bron, P ;

Kleerebezem, M ;

Wells, J .

ADVANCES IN APPLIED MICROBIOLOGY, VOL 56, 2004, 56 :1-64

[5] Lactobacillli expressing llama VHH fragments neutralise Lactococcus phages [J].

Hultberg, Anna ;

Tremblay, Denise M. ;

de Haard, Hans ;

Verrips, Theo ;

Moineau, Sylvain ;

Hammarstoem, Lennart ;

Marcotte, Harold .

BMC BIOTECHNOLOGY, 2007, 7 (1)

[6] Contribution of aggregation-promoting factor to maintenance of cell shape in Lactobacillus gasseri 4B2 [J].

Jankovic, I ;

Ventura, M ;

Meylan, V ;

Rouvet, M ;

Elli, M ;

Zink, R .

JOURNAL OF BACTERIOLOGY, 2003, 185 (11) :3288-3296

[7] In situ delivery of passive immunity by lactobacilli producing single-chain antibodies [J].

Krüger, C ;

Hu, YZ ;

Pan, Q ;

Marcotte, H ;

Hultberg, A ;

Delwar, D ;

van Dalen, PJ ;

Pouwels, PH ;

Leer, RJ ;

Kelly, CG ;

van Dollenweerd, C ;

Ma, JK ;

Hammarström, L .

NATURE BIOTECHNOLOGY, 2002, 20 (07) :702-706

[8] Therapeutic effect of llama derived VHH fragments against Streptococcus mutans on the development of dental caries [J].

Kruger, Carina ;

Hultberg, Anna ;

Marcotte, Harold ;

Hermans, Pim ;

Bezemer, Sandra ;

Frenken, Leon G. J. ;

Hammarstrom, Lennart .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 72 (04) :732-737

[9] Expression of single-chain antibody against RgpA protease of Porphyromonas gingivalis in Lactobacillus [J].

Marcotte, H ;

Koll-Klais, P ;

Hultberg, A ;

Zhao, Y ;

Gmür, R ;

Mändar, R ;

Mikelsaar, M ;

Hammarström, L .

JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (02) :256-263

[10] The aggregation-promoting factor of Lactobacillus crispatus M247 and its genetic locus [J].

Marcotte, H ;

Ferrari, S ;

Cesena, C ;

Hammarström, L ;

Morelli, L ;

Pozzi, G ;

Oggioni, MR .

JOURNAL OF APPLIED MICROBIOLOGY, 2004, 97 (04) :749-756

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