Direct synthesis of an oligonucleotide-poly(phenylene ethynylene) conjugate with a precise one-to-one molecular ratio

被引：93

作者：

Yang, CYJ ^{[1
]}

Pinto, M ^{[1
]}

Schanze, K ^{[1
]}

Tan, WH ^{[1
]}

机构：

[1] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 17期

关键词：

biosensors; conjugated polymers; fluorescent probes; molecular beacons; oligonucleotides;

D O I：

10.1002/anie.200462431

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Polymer probes light up: Amplifying fluorescent polymers conjugated with biomolecules have excellent light-harvesting and superquenching properties (see scheme). A novel synthetic method is reported for the highly efficient, simple, fast, and readily controllable conjugation of a water-soluble poly(phenylene ethynylene) with an oligonucleotide. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2572 / 2576

页数：5

共 22 条

[1] Poly(aryleneethynylene)s: Syntheses, properties, structures, and applications [J].

Bunz, UHF .

CHEMICAL REVIEWS, 2000, 100 (04) :1605-1644

[2] Imaging single fluorescent molecules at the interface of an optical fiber probe by evanescent wave excitation [J].

Fang, XH ;

Tan, WH .

ANALYTICAL CHEMISTRY, 1999, 71 (15) :3101-3105

[3] Designing a novel molecular beacon for surface-immobilized DNA hybridization studies [J].

Fang, XH ;

Liu, XJ ;

Schuster, S ;

Tan, WH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (12) :2921-2922

[4] Molecular beacons - Novel fluorescent probes [J].

Fang, XH ;

Li, JWJ ;

Perlette, J ;

Tan, WH ;

Wang, KM .

ANALYTICAL CHEMISTRY, 2000, 72 (23) :747A-753A

[5] DNA hybridization detection with water-soluble conjugated polymers and chromophore-labeled single-stranded DNA [J].

Gaylord, BS ;

Heeger, AJ ;

Bazan, GC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (04) :896-900

[6] DNA detection using water-soluble conjugated polymers and peptide nucleic acid probes [J].

Gaylord, BS ;

Heeger, AJ ;

Bazan, GC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (17) :10954-10957

[7] Making sense of polymer-based biosensors [J].

Heeger, PS ;

Heeger, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12219-12221

[8]

Huang H., 2004, ANGEW CHEM INT EDIT, V116, P5753, DOI [10.1002/ange.200460371, DOI 10.1002/ANGE.200460371]

[9] Design of a modular-based fluorescent conjugated polymer for selective sensing [J].

Huang, HM ;

Wang, K ;

Tan, WH ;

An, D ;

Yang, XH ;

Huang, SS ;

Zhai, Q ;

Zhou, L ;

Jin, Y .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (42) :5635-5638

[10] Superquenching and its applications in J-aggregated cyanine polymers [J].

Jones, RM ;

Bergstedt, TS ;

Buscher, CT ;

McBranch, D ;

Whitten, D .

LANGMUIR, 2001, 17 (09) :2568-2571

← 1 2 3 →