Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection

被引：29

作者：

Zeng, Ruoyun ^{[1
,2
]}

Pan, Weiqiang ^{[1
]}

Lin, Yifan ^{[2
]}

He, Jian ^{[1
]}

Luo, Zhiyong ^{[1
]}

Li, Zhimin ^{[1
]}

Weng, Shaoping ^{[2
]}

He, Jianguo ^{[1
,2
]}

Guo, Changjun ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, State Key Lab Biocontrol,Higher Educ Mega Ctr, Southern Marine Sci & Engn Guangdong Lab Zhuhai,S, Guangdong Prov Key Lab Marine Resources & Coastal, 132 Waihuan Dong Rd, Guangzhou 510006, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, 135 Xingang Rd West, Guangzhou 510275, Peoples R China

来源：

ISCIENCE | 2021年 / 24卷 / 07期

基金：

中国国家自然科学基金;

关键词：

KIDNEY NECROSIS VIRUS; MAJOR CAPSID PROTEIN; INFECTIOUS SPLEEN; MANDARIN FISH; IMMUNITY; DISEASE;

D O I：

10.1016/j.isci.2021.102750

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aquaculture provides important food, nutrition, and income sources for humans. However, aquaculture industry is seriously threatened by viral diseases. Infectious spleen and kidney necrosis virus (ISKNV) disease causes high mortality and economic losses to the fish culture industry in Asia and has been listed as a certifiable disease by the International Epizootic Office. Vaccine development is urgent to control this disease. Here, a gene-deleted live attenuated candidate vaccine (Delta ORF022L) against ISKNV with low pathogenicity and high protection was developed. Delta ORF022L replicated well inmandarin fish fry-1 cells and showed similar structure with wild-type ISKNV. However, the pathogenicity was significantly lower as 98% of the mandarin fish infected with Delta ORF022L survived, whereas all those infected with wild-type ISKNV died. Of importance, 100% of the Delta ORF022L-infected fish survived the ISKNV challenge. Delta ORF022L induced anti-ISKNV specific antibody response and upregulation of immune-related genes. This work could be beneficial to the control of fish diseases.

引用

页数：17

共 35 条

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