Axon regeneration in goldfish and rat retinal ganglion cells: Differential responsiveness to carbohydrates and cAMP

被引:0
作者
Li, YM
Irwin, N
Yin, YQ
Lanser, M
Benowitz, LI
机构
[1] Childrens Hosp, Labs Neurosci Res Neurosurg, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Program Neurosci, Boston, MA 02115 USA
[4] Boston Life Sci Inc, Boston, MA 02115 USA
关键词
retina; optic nerve; axon; regeneration; retinal ganglion cells; mannose; growth factors; macrophages; cAMP;
D O I
暂无
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Mammalian retinal ganglion cells (RGCs) do not normally regenerate their axons through an injured optic nerve, but can be stimulated to do so by activating macrophages intraocularly. In a cell culture model of this phenomenon, we found that a small molecule that is constitutively present in the vitreous, acting in concert with macrophage-derived proteins, stimulates mature rat RGCs to regenerate their axons if intracellular cAMP is elevated. In lower vertebrates, RGCs regenerate their axons spontaneously in vivo, and in culture, the most potent axon-promoting factor for these cells is a molecule that resembles the small vitreous-derived growth factor from the rat. This molecule was isolated chromatographically and was shown by mass spectrometry to be a carbohydrate. In agreement with this finding, D-mannose proved to be a potent axon-promoting factor for rat RGCs (ED50 similar to 10 muM); this response was cAMP-dependent and was augmented further by macrophage-derived proteins. Goldfish RGCs showed far less selectivity, responding strongly to either D-mannose or D-glucose in a cAMP-independent manner. These findings accord well with the success or failure of optic nerves to regenerate in higher and lower vertebrates in vivo. The axon-promoting effects of mannose are highly specific and are unrelated to energy metabolism or glycoprotein synthesis.
引用
收藏
页码:7830 / 7838
页数:9
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