Functionalized thermosensitive latex particles:: Useful tools for diagnostics

被引:25
作者
Pichot, C [1 ]
Taniguchi, T [1 ]
Delair, T [1 ]
Elaïssari, A [1 ]
机构
[1] Ecole Normale Super Lyon, CNRS, Unite Mixte bioMerieux, F-69364 Lyon, France
关键词
thermopositive particles; synthesis; biomolecules; diagnostic;
D O I
10.1081/DIS-120021799
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
This paper aims at reviewing recent work dedicated in this lab to the elaboration and applications in the biomedical domain of hydrophilic stimuli-responsive latex particles based on the use of various polyalkyl(meth)acrylamides together with functional monomers bearing bioreactive groups. At first, a brief description is provided on the synthesis of such particles, differing in their surface and internal morphology, through radical-initiated polymerization processes in heterogeneous media. Then, it is shown that several colloidal properties of these particles drastically change, not only as a function of temperature, but also vs. pH and salinity. Knowledge of these characteristics is a prerequisite for optimal control of the immobilization of various biologically-active macromolecules, mostly by covalent binding. Finally, some applications of these particles are described, especially for improving sensitivity in gene diagnostics or for the separation and concentration of biomolecules.
引用
收藏
页码:423 / 437
页数:15
相关论文
共 30 条
[1]   Study of cationic N-isopropylacrylamide-styrene copolymer latex particles using fluorescent probes [J].
Castanheira, EMS ;
Martinho, JMG ;
Duracher, D ;
Charreyre, MT ;
Elaïssari, A ;
Pichot, C .
LANGMUIR, 1999, 15 (20) :6712-6717
[2]  
CHARLES MH, 2001, STP PHARM, V11, P241
[3]   Amino-containing cationic latex-oligodeoxyribonucleotide conjugates:: application to diagnostic test sensitivity enhancement [J].
Delair, T ;
Meunier, F ;
Elaïssari, A ;
Charles, MH ;
Pichot, C .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1999, 153 (1-3) :341-353
[4]   Adsorption of modified HIV-1 capsid p24 protein onto thermosensitive and cationic core-shell poly(styrene)-poly(N-isopropylacrylamide) particles [J].
Duracher, D ;
Elaïssari, A ;
Mallet, F ;
Pichot, C .
LANGMUIR, 2000, 16 (23) :9002-9008
[5]  
Duracher D, 1999, J POLYM SCI POL CHEM, V37, P1823
[6]   Cationic amino-containing N-isopropylacrylamide-styrene copolymer latex particles: 1 - Particle size and morphology vs. polymerization process [J].
Duracher, D ;
Sauzedde, F ;
Elaissari, A ;
Perrin, A ;
Pichot, C .
COLLOID AND POLYMER SCIENCE, 1998, 276 (03) :219-231
[7]   Cationic amino-containing N-isopropyl-acrylamide-styrene copolymer particles: 2-surface and colloidal characteristics [J].
Duracher, D ;
Sauzedde, F ;
Elaissari, A ;
Pichot, C ;
Nabzar, L .
COLLOID AND POLYMER SCIENCE, 1998, 276 (10) :920-929
[8]  
DURACHER D, 2000, MACROMOLECULAR S POL, V151, P297
[9]  
DURACHER D, 1999, THESIS LYON
[10]   Hydrophilic magnetic latex for nucleic acid extraction, purification and concentration [J].
Elaïssari, A ;
Rodrigue, M ;
Meunier, F ;
Herve, C .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2001, 225 (1-2) :127-133