Self Nano Emulsifying Drug Delivery System (SNEDDS) Activity of Pegagan (Centella asiatica L) Extraction on Zebrafish Caudal Fins Regeneration

Centella asiatica L. is a herbal medicine with antioxidant, antihyperglycemic, and wound-healing effects. It contains asiatic acid, madecassic acid, asiaticoside, and madecassoside. Asiatic acid in C. asiatica is a non-polar compound, thus it is necessary to overcome the problem of solubility prepared in the SNEDDS formula. This study was conducted to determine the effect of SNEDDS preparations on C. asiatica extract on the regeneration of zebrafish caudal fins. The test was conducted using zebra fish divided into 6 groups (n = 8): negative control (amputated without treatment), solvent control (amputated and given SNEDDS without C. asiatica extract), treatment group (amputated and given SNEDDS C. asiatica extract doses 5, 10, and 20 ppm), and normal group (not amputated and not treated). The regeneration of caudal fins was observed using a stereo microscope for 28 days. The results of the calculation of regeneration areas were analyzed using one-way ANOVA (p<0.05). The result showed that the percentage calculation of regeneration areas are 56.19% (negative control); 53.74% (solvent control); 81.09% (5 ppm SNEDDS); 54.04% (10 ppm SNEDDS); and 56.70% (20 ppm SNEDDS). SNEDDS C. asiatica extracts 5 ppm have a better regeneration area than others (p<0.05). SNEDDS C. asiatica extract dose of 5 ppm is proven to have the best wound healing activity in the process of regeneration of zebrafish caudal fins.