Effects of vascular formation during alveolar bone process morphogenesis in mice

被引:13
作者
An, Seo-Young [1 ]
Lee, Ye-Ji [2 ]
Neupane, Sanjiv [2 ]
Jun, Jong-Hwa [3 ]
Kim, Ji-Youn [4 ]
Lee, Youngkyun [2 ]
Choi, Karp-Shik [1 ]
An, Chang-Hyeon [1 ]
Suh, Jo-Young [5 ]
Shin, Hong-In [6 ]
Sohn, Wern-Joo [2 ]
Kim, Jae-Young [2 ]
机构
[1] Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Radiol, IHBR, Daegu, South Korea
[2] Kyungpook Natl Univ, Sch Dent, Dept Biochem, IHBR, 2177 Dalgubeol Daero, Daegu 41940, South Korea
[3] Keimyung Univ, Dept Ophthalmol, Daegu, South Korea
[4] Gachon Univ, Dept Dent Hyg, Incheon, South Korea
[5] Kyungpook Natl Univ, Sch Dent, Dept Periodontol, IHBR, Daegu, South Korea
[6] Kyungpook Natl Univ, Sch Dent, Dept Oral & Maxillofacial Pathol, IHBR, Daegu, South Korea
关键词
Alveolar bone process; VEGF; Periodontium; Bevacizumab; Microinjection model; ENDOTHELIAL GROWTH-FACTOR; SMOOTH MUSCLE ACTIN; FACTOR MONOCLONAL-ANTIBODY; PERIODONTAL-LIGAMENT; IMMUNOHISTOCHEMICAL LOCALIZATION; ANGIOGENESIS; TOOTH; CELLS; EXPRESSION; BIOLOGY;
D O I
10.1007/s00418-017-1584-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The alveolar bone process is the thickened ridge of bone that bears the teeth and is known to have dynamic functional interactions with surrounding tissues. However, the detailed morphological changes that occur during alveolar bone process development and the underlying molecular mechanisms behind this morphogenesis have not been elucidated. In this study, we examined the detailed morphological changes of the alveolar bone process during mouse development using HE and MTC staining. In addition, we evaluated the precise localization pattern of various signaling molecules involved in blood vessel formation including CD31, alpha-SMA, VEGF, periostin, and TGF-beta. Innervation of the alveolar bone process was examined following injection of the nerve terminal dye AM1-43. The morphological and immunohistochemical data suggested that there is an intimate relationship between alveolar bone process development and blood vessel formation. To more closely examine the role of blood vessels in alveolar bone process formation, we microinjected mice with a clinically available anti-VEGF antibody, bevacizumab, at PN5 and analyzed the effects 5 days later. Compared to the control animals, anti-VEGF treated animals showed a disruption of the integration of bony tissues to form the alveolar bone process structures, which should contain the periodontal ligaments. Based on these data, we conclude that specific morphogenesis of the alveolar bone process is closely associated with blood vessel formation.
引用
收藏
页码:435 / 443
页数:9
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