Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography

被引：3
作者：
Carvalho, RSH
Ianzer, DA
Malavolta, L
Rodrigues, MM
Cilli, EM
Nakaie, CR
机构：
[1] Univ Fed Sao Paulo, Dept Biophys, BR-04044020 Sao Paulo, Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Parasitol & Immunol, BR-04023062 Sao Paulo, Brazil
[3] Univ Estadual Paulista, Inst Chem, Dept Biochem & Technol Chem, BR-14800900 Sao Paulo, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 817卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
resin; peptide; polymer; ion-exchange chromatography; affinity chromatography;
D O I：
10.1016/j.jchromb.2004.12.008
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. The so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. In addition, the synthesized acetyl-(NANP)(3)-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：231 / 238
页数：8
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