In-vivo quantification of primary microRNA processing by Drosha with a luciferase based system

被引：18

作者：

Allegra, Danilo

Mertens, Daniel ^{[1
]}

机构：

[1] Univ Hosp, Dept Internal Med 3, D-89081 Ulm, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 406卷 / 04期

关键词：

MicroRNA; Luciferase; miRNA processing assay; Drosha; Microprocessor; miR-16; RNA; MATURATION; RECOGNITION; COMPLEX; REGION; SUBSET; DICER;

D O I：

10.1016/j.bbrc.2011.02.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RNAse III Drosha is responsible for the first step of microRNA maturation, the cleavage of primary miRNA to produce the precursor miRNA. Processing by Drosha is finely regulated and influences the amount of mature microRNA in a cell. We describe in the present work a method to quantify Drosha processing activity in-vivo, which is applicable to any microRNA. With respect to other methods for measuring Drosha activity, our system is faster and scalable, can be used with any cellular system and does not require cell sorting or use of radioactive isotopes. This system is useful to study regulation of Drosha activity in physiological and pathological conditions. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：501 / 505

页数：5

共 50 条

[41] Flexible CW-fNIRS system based on Silicon Photomultipliers: In-Vivo characterization of sensorimotor response
Chiarelli, Antonio Maria
Zappasodi, Filippo
di Pompeo, Francesco
Merla, Arcangelo
Mazzillo, Massimo
Fallica, Giorgio
Libertino, Sebania
Lombardo, Salvatore
2017 IEEE SENSORS, 2017, : 1673 - 1675
[42] Localization of Uncemented Hip Stem Loosening with a Novel In-vivo Sensor System Based on Vibration Analysis
Ruther, C.
Ewald, H.
Mittelmeier, W.
Bader, R.
Kluess, D.
6TH WORLD CONGRESS OF BIOMECHANICS (WCB 2010), PTS 1-3, 2010, 31 : 620 - +
[43] Development of the in-vivo system of the cochlear implant debugging platform based on embedded pulse control mode
Jiang, Tao
Gong, Qin
Yi Qi Yi Biao Xue Bao/Chinese Journal of Scientific Instrument, 2015, 36 (07): : 1673 - 1680
[44] Flexible manufacturing control system in RCS based tobacco primary processing
Che, Guo-Lin
Lai, Hua
Zhongnan Daxue Xuebao (Ziran Kexue Ban)/Journal of Central South University (Science and Technology), 2011, 42 (SUPPL. 1): : 1119 - 1122
[45] Primary results of in-vivo needle-based confocal laser endomicroscopy in human prostate: a safety and feasibility study
Van Riel, Luigi
Swaan, Abel
Mannaerts, Chrisopthe
Savci-Heijink, Dilara
Van Leeuwen, Ton
De Reijke, Theo
De Bruin, Martijn
INTERNATIONAL JOURNAL OF UROLOGY, 2020, 27 : 11 - 11
[46] In-vivo proximal monitoring system for plant water stress and biological activity based on stem electrical impedance
Calvo, Stefano
Barezzi, Mattia
Demarchi, Danilo
Garlando, Umberto
2023 9TH INTERNATIONAL WORKSHOP ON ADVANCES IN SENSORS AND INTERFACES, IWASI, 2023, : 80 - 85
[47] A three dimensional real-time MEMS based optical biopsy system for in-vivo clinical imaging
McCormick, Daniel T.
Jung, Woonggyu
Ahn, Yeh-Chan
Chen, Zhongping
Tien, Norman C.
TRANSDUCERS '07 & EUROSENSORS XXI, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2, 2007,
[48] Quantification of Choroidal Blood Flow Using the OCT-A System Based on Voxel Scan Processing
Iomdina, Elena N.
Kiseleva, Anna A.
Kotliar, Konstantin E.
Luzhnov, Petr, V
PROCEEDINGS OF THE 2020 INTERNATIONAL CONFERENCE ON BIOMEDICAL INNOVATIONS AND APPLICATIONS (BIA 2020), 2020, : 41 - 44
[49] An Intelligent Metallographic Processing System for Identifying Primary Dendrites Based on Deep Learning
Cui, L. J.
Sun, M. Y.
Guo, S. R.
Cao, Y. L.
Zeng, W. H.
Li, X. L.
Zheng, B.
LASERS IN ENGINEERING, 2021, 48 (4-6) : 331 - 347
[50] An intelligent metallographic processing system for identifying primary dendrites based on deep learning
Cui, L.J.
Sun, M.Y.
Guo, S.R.
Cao, Y.L.
Zeng, W.H.
Li, X.L.
Zheng, B.
Lasers in Engineering, 2021, 48 (04): : 331 - 347

← 1 2 3 4 5 →