New stationary phase for anion-exchange chromatography

被引:81
作者
Auler, LMLA
Silva, CR
Collins, KE
Collins, CH
机构
[1] Univ Estadual Campinas, LABCROM, Lab Pesquisas Cromatog Liquida, Inst Quim, BR-13084971 Campinas, SP, Brazil
[2] Comis Nacl Energia Nucl, Ctr Desenvolvimento Tecnol Nucl, BR-30123970 Belo Horizonte, MG, Brazil
关键词
propylpyridinium; silica; anion chromatography; stationary phase;
D O I
10.1016/j.chroma.2004.10.012
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This work describes the preparation of an anion-exchange phase based on silica, using a two-step modification process. First, 10 mu m Davisil silica particles were silanized with chloropropyltrimethoxysilane to yield chloropropyl silica. The modified silica was then reacted with pyridine to produce positively charged propylpyridinium groups on the surface, the anion-exchange sites. The phase was characterized by thermogravimetric analysis and infrared and solid state C-13 and Si-29 NMR spectroscopies. HPLC separations of common inorganic anions, including chloride, nitrite, bromide and nitrate, were performed using 150 x 3.9 HPLC columns packed with the phase, using a phthalate buffer solution as mobile phase with non-suppressed conductivity detection. Efficiency and resolution were calculated and the results show that the new phase has significant promise for the analysis of these anions in environmental samples. (c) 2004 Elsevier B.V All rights reserved.
引用
收藏
页码:147 / 153
页数:7
相关论文
共 24 条
[1]   CHARACTERIZATION OF BONDED PHASES BY SOLID-STATE NMR-SPECTROSCOPY [J].
ALBERT, K ;
BAYER, E .
JOURNAL OF CHROMATOGRAPHY, 1991, 544 (1-2) :345-370
[2]   Synthesis and solid-state NMR investigations of radiation-immobilized polysiloxanes on bare, titanium-grafted, and zirconium-grafted silicas [J].
Bachmann, S ;
Melo, LFC ;
Silva, RB ;
Anazawa, TA ;
Jardim, ICSF ;
Collins, KE ;
Collins, CH ;
Albert, K .
CHEMISTRY OF MATERIALS, 2001, 13 (05) :1874-1879
[3]   Effect of buffers on silica-based column stability in reversed-phase high-performance liquid chromatography [J].
Claessens, HA ;
vanStraten, MA ;
Kirkland, JJ .
JOURNAL OF CHROMATOGRAPHY A, 1996, 728 (1-2) :259-270
[4]  
Collins KE, 2000, LC GC N AM, V18, P106
[5]  
Collinson MM, 2002, TRAC-TREND ANAL CHEM, V21, P30
[6]   Fast separation of UV absorbing anions using ion-interaction chromatography [J].
Connolly, D ;
Paull, B .
JOURNAL OF CHROMATOGRAPHY A, 2001, 917 (1-2) :353-359
[7]   Rapid, low pressure, and simultaneous ion chromatography of common inorganic anions and cations on short permanently coated monolithic columns [J].
Connolly, D ;
Victory, D ;
Paull, B .
JOURNAL OF SEPARATION SCIENCE, 2004, 27 (10-11) :912-920
[8]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF INORGANIC AND ORGANIC-ANIONS USING ULTRAVIOLET DETECTION AND AN AMINO COLUMN [J].
CORTES, HJ .
JOURNAL OF CHROMATOGRAPHY, 1982, 234 (02) :517-520
[9]   ALKOXY SILANES FOR THE PREPARATION OF SILICA BASED STATIONARY PHASES WITH BONDED POLAR FUNCTIONAL-GROUPS [J].
ENGELHARDT, H ;
ORTH, P .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1987, 10 (8-9) :1999-2022
[10]   Adsorption of FeCl3, CuCl2 and ZnCl2 on silsesquioxane 3-n-propylpyridiniumchloride polymer film adsorbed on Al2O3 coated silica gel [J].
Fujiwara, ST ;
Gushikem, Y ;
Alfaya, RVS .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2001, 178 (1-3) :135-141