Incorporating Tenax into the in vitro method to improve the predictive capability of bioaccessibility of triazole fungicides in grape

In vitro bioaccessibility assays have been developed for high-throughput prediction of relative bioavailability (RBA). However, methods to reliably and efficiently assess pesticide residues remain limited, hindering the precise estimation of pesticide exposure risk. The inclusion of a sorption sink material to simulate intestinal sorption could be a promising approach to optimize in vitro bioaccessibility methods. The current study aimed to explore the feasibility of incorporating Tenax into the Rijksinstituut voor Volksgezondheid en Milieu (RIVM) method for accurate evaluation of the bioaccessibility of triazole fungicides. The use of 1.0 g of Tenax enabled the valid trapping of triazole fungicides released from grape, resulting in a significant increase of 23.59-38.51 % in the value of bioaccessibility. A strong in vivo-in vitro correlation was observed between pesticide RBA and bioaccessibility, suggesting that the Tenax-assisted RIVM method is a suitable replacement for time-consuming and laborious in vivo alternatives. In addition, the exposure assessment indicated that the hazard quotients for triazole fungicides in grape may be overestimated by 5.79-27.34 % without considering bioaccessibility based on the Tenax-assisted RIVM method. These results provide further insights into the assessment of bioaccessibility-based human exposure to pesticides as well as dietary exposure and related risk for human health.