Affinity chromatography of porcine pepsin on different types of immobilized 3,5-diiodo-L-tyrosine

被引：18

作者：

Arnostová, H

Kucerová, Z

Tislerová, I

Trnka, T

Tichá, M

机构：

[1] Charles Univ, Dept Biochem, Prague 12840, Czech Republic

[2] Charles Univ, Dept Pathophysiol, Prague 12853, Czech Republic

[3] Charles Univ, Dept Organ Chem, Prague 12840, Czech Republic

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 911卷 / 02期

关键词：

immobilized amino acids; pepsin; diiodotyrosine; enzymes;

D O I：

10.1016/S0021-9673(00)01255-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of affinity sorbents containing immobilized iodinated derivatives of L-tyrosine for the affinity chromatography of porcine pepsin is described. The ligand was coupled either to Sepharose 4B or bead cellulose after the divinylsulfone activation or to Sepharose 4B after the activation with 2,4,6-trichloro-1,3,5-triazine. The highest capacity for porcine pepsin was found in the case of 3,5-diiodo-L-tyrosine coupled to divinylsulfone-activated Sepharose. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：211 / 216

页数：6

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