Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds

被引：43

作者：

Sun Jun-ming ^{[1
]}

Sun Bao-li ^{[2
]}

Han Fen-xia ^{[1
]}

Yan Shu-rong ^{[1
]}

Yang Hua ^{[1
]}

Kikuchi, Akio ^{[3
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[2] Chinese Acad Agr Sci, Inst Agr Environm & Sustainable Dev, Beijing 100081, Peoples R China

[3] Natl Agr Res Ctr Tohoku Reg, Akita 0192112, Japan

来源：

AGRICULTURAL SCIENCES IN CHINA | 2011年 / 10卷 / 01期

基金：

中国国家自然科学基金;

关键词：

soybean (Glycine max L. Merri); isoflavone; HPLC; RESISTANCE;

D O I：

10.1016/S1671-2927(11)60308-8

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

It is important to determine the isoflavone components by high-performance liquid chromatography (HPLC) for the molecular assistant selection of isoflavone in soybean. Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC. The results showed that there were different maximum ultraviolet (UV) absorbance for the aglycones of daidzein, glycitein, and genistein, which were at 250, 257, and 260 nm, respectively. A linear gradient elution of acetonitrile (13-30%) containing 0. 1% acetic acid as a mobile phase was applied on a YMC-C18 column at 35 degrees C. The 12 isoflavone components were determined using the UV detector by HPLC. We concluded that this is a rapid and precise method which adapted to determine the large numbers of samples with microanalysis.

引用

页码：70 / 77

页数：8

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