A single chain Fv antibody displayed on phage surface recognises conformational group-specific epitope of bluetongue virus

被引：6

作者：

Nagesha, HS

Wang, LF

Shiell, B

Beddome, G

White, JR

Irving, RA

机构：

[1] CSIRO, Australian Anim Hlth Lab, Geelong, Vic 3220, Australia

[2] CRC, Div Hlth Sci & Nutr, CSIRO, Parkville, Vic 3052, Australia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2001年 / 91卷 / 02期

关键词：

ScFv; filamentous phage; coat protein; bluetongue virus;

D O I：

10.1016/S0166-0934(00)00266-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A single chain fragment variable (scFv) antibody gene was isolated from hybridoma cell line secreting monoclonal antibody (MAb) 20E9 that recognises bluetongue virus (BTV) VP7. DNA fragments encoding variable regions of heavy and light chains were amplified by RT-PCR and library of scFv was constructed in phage vector. Two scFv clones that were selected showed specific reactivity with conformational epitope VP7. The N-terminal 22 amino acid residues of 20E9 light chain were identical to that deduced from scFv DNA sequence. An in-frame TAG stop codon was found in the coding sequence and its potential role in regulating the expression and stability of scFv in phage is discussed. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 207

页数：5

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