Brazilin and the extract from Caesalpinia sappan L. protect oxidative injury through the expression of heme oxygenase-1

被引：33

作者：

Choi, Byung-Min ^{[1
,2
]}

Lee, Ju-A. ^{[1
,2
]}

Gao, Shang Shang ^{[1
,2
]}

Eun, Sang Yong ^{[1
,2
]}

Kim, Young-Sup ^{[3
]}

Ryu, Shi-Yong

Choi, Yeon-Hee

Park, Raekil ^{[2
,4
]}

Kwon, Dae Young ^{[5
]}

Kim, Bok-Ryang ^{[1
,2
]}

机构：

[1] Wonkwang Univ, Dept Biochem, Sch Med, Iksan 570749, Chonbuk, South Korea

[2] Wonkwang Univ, Vestibulocochlear Res Ctr, Sch Med, Iksan 570749, Chonbuk, South Korea

[3] Korea Res Inst Chem Technol, Phytochem Res Lab, Taejon 305606, South Korea

[4] Wonkwang Univ, Dept Microbiol, Sch Med, Iksan 570749, Chonbuk, South Korea

[5] Korea Food Res Inst, Funct Food Res Ctr, Gyungki Do, South Korea

来源：

BIOFACTORS | 2007年 / 30卷 / 03期

关键词：

Caesalpinia sappan; brazilin; heme oxygenase-1; oxidative stress; zinc protoporphyrin IX;

D O I：

10.1002/biof.5520300302

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we examined the protective effects of Caesalpinia sappan L. and its major component, brazilin, against tert-butylhydroperoxide (t-BHP)-induced cell death in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells. We found that the extract of C. sappan L. and brazilin induced antioxidant response element (ARE)-luciferase activity and heme oxygenase-1 (HO-1) expression in a concentration-dependent manner. The inductive effect of brazilin was more potent than the extract of C. sappan L. and the expression of HO-1 reached a peak at 12 h after brazilin treatment. The extract and brazilin protected the cells against t-BHP-induced cell death. Their protective effects were abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results demonstrate that the extract of C. sappan L. and brazilin induce the expression of HO-1 and the enzyme diminishes t-BHP-induced cell death in HEI-OC1 cells.

引用

页码：149 / 157

页数：9

共 34 条

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