Physiology, pathogenicity and immunogenicity of live, attenuated Salmonella enterica serovar Enteritidis mutants in chicks

被引：7

作者：

Si, Wei ^{[1
]}

Wang, Xiumei ^{[1
]}

Liu, Huifang ^{[1
]}

Yu, Shenye ^{[1
]}

Li, Zhaoli ^{[1
]}

Chen, Liping ^{[1
]}

Zhang, Wanjiang ^{[1
]}

Liu, Siguo ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, Div Bacterial Dis, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

来源：

MICROBIAL PATHOGENESIS | 2015年 / 83-84卷

关键词：

Chicks; Live attenuated vaccines; S; Enteritidis; Immune response; COMPETITIVE-EXCLUSION; VACCINE; IMMUNIZATION; TYPHIMURIUM; PROTEASE; GENE;

D O I：

10.1016/j.micpath.2015.03.018

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To construct a novel live, attenuated Salmonella vaccine, the Ion, cpxR and cpdB genes were deleted from a wild-type Salmonella enterica serovar Enteritidis-6 (SM-6) strain using the phage gimel Red homologous recombination system, resulting in SM-Delta CpxR, SM-Delta C/Lon and SM-Delta C/L/CpdB. The growth curves of strain SM-Delta C/Lon grew more rapidly than the other strains and had OD 600 values higher than the other strains starting at the 4 h time point. The growth curves of strain SM-Delta C/L/CpdB were relatively flat. The colonization time of SM-Delta C/L/CpdB is about 8-10 days. Deleting the lon/cpxR/cpdB (SM-6) genes resulted in an approximate 10(3)-fold attenuation in virulence assessed by the analysis of the LD50 of specific pathogen-free (SPF) chicks. This result indicated that the deletion of the Ion, cpxR and cpdB genes induced significant virulence attenuation. The protective effects of SM-Delta C/L/CpdB vaccination in SPF chicks against 5 x 10(9) colony forming units (CFU) of S. Enteritidis were resulted from the induction of an effective immune response. These findings demonstrate the potential of mutant SM-Delta C/L/CpdB to be used as an effective vaccine. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：6 / 11

页数：6

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