A new diterpenoid glycoside, 13-[(2-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-(4-O-(2-O-alpha-D-glucopyranosyl)-alpha-D-glucopyranosyl-beta-D-glucopyranosyl) ester (1) was isolated from the commercial extract of the leaves of Stevia rebaudiana along with the known steviol glycosides including stevioside, rebaudiosides A-G, rubusoside and dulcoside A. The complete H-1 and C-13 NMR assignments of the new compound and rebaudioside G (2) were achieved by the extensive NMR (H-1 and C-13, COSY, 1D TOCSY, HSQC, HMBC) and MS spectral data as well as chemical studies.