Hybrid organic-inorganic phenyl monolithic column for capillary electrochromatography

被引：78
作者：
Yan, LJ
Zhang, QH [1 ]
Zhang, WB
Feng, YQ
Zhang, LH
Li, T
Zhang, YK
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
[2] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
[3] Dalian Elite Analyt Instruments Co Ltd, Dalian, Peoples R China
来源：
ELECTROPHORESIS | 2005年 / 26卷 / 15期
关键词：
capillary electrochromatography; monolithic columns; organic-inorganic hybrid materials; sol-gel; stationary phases;
D O I：
10.1002/elps.200500016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel hybrid organic-inorganic silica-based monolithic column possessing phenyl ligands for reversed-phase (RP) capillary electrochromatography (CEC) is described. The monolithic stationary phase was prepared by in situ co-condensation of tetraethoxysilane (TEOS) with phenyltriethoxysilane (PTES) via a two-step catalytic sol-gel procedure to introduce phenyl groups distributed throughout the silica matrix for chromatographic interaction. The hydrolysis and condensation reactions of precursors were chemically controlled through pH variation by adding hydrochloric acid and dodecylamine, respectively. The structural property of the monolithic column can be easily tailored through adjusting the composition of starting sol solution. The effect of PTES/TEOS ratios on the morphology of the created stationary phases was investigated. A variety of neutral and basic analytes were used to evaluate the column performance. The CEC columns exhibited typical RP chromatographic retention mechanism for neutral compounds and had improved peak shape for basic solutes.
引用
收藏
页码：2935 / 2941
页数：7
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