Enhanced stability of Candida antarctica lipase B in ionic liquids

被引：33

作者：

Ha, Sung Ho ^{[1
]}

Lee, Sang Hyun ^{[1
]}

Dang, Dung Thanh ^{[2
]}

Kwon, Min Sik ^{[2
]}

Chang, Woo-Jin ^{[1
]}

Yu, Yong Jae ^{[3
]}

Byun, Il Suk ^{[3
]}

Koo, Yoon-Mo ^{[1
,2
]}

机构：

[1] Inha Univ, ERC Adv Bioseparat Technol, Inchon 402751, South Korea

[2] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[3] CTRI, Hwaseong, Gyeonggi Do, South Korea

来源：

KOREAN JOURNAL OF CHEMICAL ENGINEERING | 2008年 / 25卷 / 02期

关键词：

ionic liquid; lipase; (RS)-1-Phenylethanol; reuse; stability;

D O I：

10.1007/s11814-008-0051-0

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The activity and stability of lipase from Candida antarctica were investigated in the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate using ionic liquids (ILs) as reaction media. Among ILs tested, the highest activity of lipase was observed in [Edmim][Tf2N. In hydrophobic ILs such as [Edmim][Tf2N], [Emim][Tf2N] and [Pmim] [PF6], lipase could retain its activity after 5 times reuse, while the activity of lipase in hydrophilic ILs and organic solvents was drastically decreased. The activities of lipase in [Edmim][Tf2N], [Emim][Tf2N] and [Pmim][PF6] were also well maintained after I day incubation at 80 degrees C. The lipase suspended in [Edmim] [Tf2N] could be successfully reused 6 times without loss of activity.

引用

页码：291 / 294

页数：4

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