Site-Specific Infrared Probes of Proteins

被引:154
|
作者
Ma, Jianqiang [1 ]
Pazos, Ileana M. [2 ]
Zhang, Wenkai [1 ]
Culik, Robert M. [3 ]
Gai, Feng [1 ,2 ]
机构
[1] Univ Penn, Ultrafast Opt Proc Lab, Philadelphia, PA 19104 USA
[2] Univ Penn, Dept Chem, Philadelphia, PA 19104 USA
[3] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
关键词
infrared spectroscopy; site-specific probe; vibrational coupling; isotope editing; unnatural amino acid; vibrational Stark effect spectroscopy; 2D IR SPECTROSCOPY; STARK-EFFECT SPECTROSCOPY; CARBON-DEUTERIUM BONDS; ELECTRIC-FIELDS; ACTIVE-SITE; AMIDE-I; VIBRATIONAL SPECTROSCOPY; SIDE-CHAIN; TEMPERATURE-JUMP; AMINO-ACIDS;
D O I
10.1146/annurev-physchem-040214-121802
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Infrared spectroscopy has played an instrumental role in the study of a wide variety of biological questions. However, in many cases, it is impossible or difficult to rely on the intrinsic vibrational modes of biological molecules of interest, such as proteins, to reveal structural and environmental information in a site-specific manner. To overcome this limitation, investigators have dedicated many recent efforts to the development and application of various extrinsic vibrational probes that can be incorporated into biological molecules and used to site-specifically interrogate their structural or environmental properties. In this review, we highlight recent advancements in this rapidly growing research area.
引用
收藏
页码:357 / 377
页数:21
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