Flavobacterium tyrosinilyticum sp nov., isolated from the rhizosphere of wild strawberry

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterial strain, designated THG-DN8.8(T), was isolated from the rhizosphere of a wild strawberry plant, located on Gyeryong Mountain, Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Flavobacterium and was related most closely to Flavobacterium banpakuense 15F3(T) (97.7% sequence similarity), Flavobacterium ginsenosidimutans THG-01(T)(97.3%), Flavobacterium ginsengiterrae DCY55(T) (96.9%) and Flavobacterium chungbukense CS100(T) (96.7%). Catalase and oxidase tests were positive. Levels of DNA-DNA relatedness with its phylogenetically closest neighbours were below 40.0%. The DNA G+C content was determined to be 39.1 mol%. Strain THG-DN8.8(T) also was found to be able to grow at 4-33 degrees C, with 0-2 % (w/v) NaCl and at pH 5.5-9.5. The major fatty acids were iso-C-15:0, summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c) and C-16:0. Menaquinone-6 (MK-6) was the dominant respiratory quinone, and phosphatidylethanolamine and phosphatidyldiethanolamine were the main polar lipids. On the basis of the data presented, strain THG-DN8.8(T) represents a novel species, for which the name Flavobacterium tyrosinilyticum sp. nov. is proposed. The type strain is THG-DN8.8(T) (=KCTC 42726(T)=CCTCC AB 2015295(T)).