NMDA receptor-mediated immediate Ser831 phosphorylation of GluR1 through CaMKIIα in rat hippocampus during early global ischemia

被引：16
作者：
Fu, XZ [1 ]
Zhang, QG [1 ]
Meng, FJ [1 ]
Zhang, GY [1 ]
机构：
[1] Xuzhou Med coll, Res Ctr Biochem & Mol Biol, Xuzhou 221002, Jiangsu, Peoples R China
来源：
NEUROSCIENCE RESEARCH | 2004年 / 48卷 / 01期
基金：
中国国家自然科学基金;
关键词：
alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor; GluR1; N-methyl-D-aspartate receptor; calcium/calmodulin-dependent kinase II alpha phosphorylation; ischemia;
D O I：
10.1016/j.neures.2003.09.009
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The phosphorylation of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors subunit GluR1 at Ser831 has been implicated in the regulation of AMPA receptors channel. In this paper, Ser831 phosphorylation of GluR1 in rat hippocampus was investigated, which significantly increased during early global ischemia. To further illustrate the underlying mechanisms, calcium/calmodulin-dependent kinase IIalpha (CaMKIIalpha) inhibitor 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine (KN62), CaM antagonist trifluoperazine (TFP), N-methyl-D-aspartate (NMDA) receptor antagonist dextromethorphan (DEX), AMPA receptor antagonist 6,7-dinitroquinoxaline-2,3-(1H,4H)-dione (DNQX) and L-type voltage-gated Ca2+ channel (L-VGCC) blocker nifedipine (NIF), were respectively administrated to the rats 20 min prior to ischemia. The results showed that KN62, TFP and DEX significantly attenuated Ser831 phosphorylation of GluR I. while DNQX and NIF had no obvious effects. Consequently, the studies suggest that early global ischemia induced Ser831 phosphorylation of GluR1 may be closely associated with CaMKIIalpha and the NMDA receptor, while the immediate Ser831 phosphorylation of GluR I may have been involved in pathogenic events after early global ischemia. (C) 2003 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.
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收藏
页码：85 / 91
页数：7
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