Analysis of metabolites of glucose pathways in human erythrocytes by analytical isotachophoresis

被引：9
|
作者：
Renner, S [1 ]
Prohaska, V [1 ]
Gerber, C [1 ]
Niethammer, D [1 ]
Bruchelt, G [1 ]
机构：
[1] Univ Tubingen, Childrens Hosp, D-72076 Tubingen, Germany
来源：
JOURNAL OF CHROMATOGRAPHY A | 2001年 / 916卷 / 1-2期
关键词：
isotachophoresis; glucose; adenosine 5 '-triphosphate; adenosine 5 '-diphosphate; lactate; pyruvate; diphosphogylcerate; organophosphorus compounds; carbohydrates;
D O I：
10.1016/S0021-9673(00)01173-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study was to establish an isotachophoretic (ITP) method for the determination of the main compounds of glycolysis in human erythrocytes in order to analyze the influence of different glucose concentrations (mimicking the situation in diabetes mellitus) on this pathway. Samples for TTP were prepared by isolation of erythrocytes. lysis of the eels by heating in double-distilled water and subsequent ultrafiltration (M-r cut-off: 5000). Ah the main compounds of glycolysis were characterized by ITP. The influence of different glucose concentrations on the main compounds of the energy metabolism (ATP, ADP, lactate, pyruvate) and 2,3-diphosphoglycerate were analyzed in short- and long-time incubations. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：247 / 253
页数：7
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