Approaching Biomarkers of Membranous Nephropathy from a Murine Model to Human Disease

被引：22

作者：

Wu, Chia-Chao ^{[1
]}

Chen, Jin-Shuen ^{[1
]}

Huang, Ching-Feng ^{[2
]}

Chen, Chun-Chi ^{[1
]}

Lu, Kuo-Chen ^{[3
]}

Chu, Pauling ^{[1
]}

Sytwu, Huey-Kang

Lin, Yuh-Feng ^{[1
,4
]}

机构：

[1] Tri Serv Gen Hosp, Dept Med, Div Nephrol, Taipei 114, Taiwan

[2] Tri Serv Gen Hosp, Dept Pediat, Taipei 114, Taiwan

[3] Cardinal Tien Hosp, Dept Med, Div Nephrol, Taipei 231, Taiwan

[4] Taipei Med Univ, Grad Inst Clin Med, Taipei 114, Taiwan

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年

关键词：

INTRAVENOUS INJECTIONS; EPITHELIAL-CELLS; GENE-EXPRESSION; IV COLLAGEN; CATHEPSIN-D; GLOMERULONEPHRITIS; LAMININ; MICE; PATHOGENESIS; MICROARRAY;

D O I：

10.1155/2011/581928

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background. Membranous glomerulonephropathy (MN) is the most prevalent cause of nephrotic syndrome in adult humans. However, the specific biomarkers of MN have not been fully elucidated. We examined the alterations in gene expression associated with the development of MN. Methods. Murine MN was induced by cationic bovine serum albumin (cBSA). After full-blown MN, cDNA microarray analysis was performed to identify gene expression changes, and highly expressed genes were evaluated as markers both in mice and human kidney samples. Results. MN mice revealed clinical proteinuria and the characteristic diffuse thickening of the glomerular basement membrane. There were 175 genes with significantly different expressions in the MN kidneys compared with the normal kidneys. Four genes, metallothionein-1 (Mt1), cathepsin D (CtsD), lymphocyte 6 antigen complex (Ly6), and laminin receptor-1 (Lamr1), were chosen and quantified. Mt1 was detected mainly in tubules, Lamr1 was highly expressed in glomeruli, and CtsD was detected both in tubules and glomeruli. The high expressions of Lamr1 and CtsD were also confirmed in human kidney biopsies. Conclusion. The murine MN model resembled the clinical and pathological features of human MN and may provide a tool for investigating MN. Applying cDNA microarray analysis may help to identify biomarkers for human MN.

引用

页数：11

共 34 条

[1]

Andonian S, 1999, J ANDROL, V20, P415

[2]

Böttinger EP, 2002, EXP NEPHROL, V10, P93

[3] Urinary excretion of β2-microglobulin and IgG predict prognosis in idiopathic membranous nephropathy:: A validation study [J].

Branten, AJW ;

du Buf-Vereijken, PW ;

Klasen, IS ;

Bosch, FH ;

Feith, GW ;

Hollander, DA ;

Wetzels, JF .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2005, 16 (01) :169-174

[4] Idiopathic membranous glomerulonephritis [J].

Cattran, DC .

KIDNEY INTERNATIONAL, 2001, 59 (05) :1983-1994

[5] Glomerulonephritis [J].

Chadban, SJ ;

Atkins, RC .

LANCET, 2005, 365 (9473) :1797-1806

[6]

Chen JR, 2004, J BONE MINER RES, V19, pS284

[7] Experimental membranous nephropathy redux [J].

Cybulsky, AV ;

Quigg, RJ ;

Salant, DJ .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2005, 289 (04) :F660-F671

[8] Abnormal expression of glomerular basement membrane laminins in membranous glomerulonephritis [J].

Fischer, E ;

Mougenot, B ;

Callard, P ;

Ronco, P ;

Rossert, J .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2000, 15 (12) :1956-1964

[9]

FOIDART JM, 1983, AM J PATHOL, V110, P346

[10] Diagnosis and natural course of membranous nephropathy [J].

Glassock, RJ .

SEMINARS IN NEPHROLOGY, 2003, 23 (04) :324-332

← 1 2 3 4 →