DNA detection of chronic myelogenous leukemia by magnetic nanoparticles

被引：19

作者：

Jangpatarapongsa, Kulachart ^{[1
]}

Polpanich, Duangporn ^{[2
]}

Yamkamon, Vichanan ^{[3
]}

Dittharot, Yuranun ^{[1
]}

Peng-On, Jutharat ^{[1
]}

Thiramanas, Raweewan ^{[2
]}

Hongeng, Suradej ^{[4
]}

Jootar, Saengsuree ^{[5
]}

Charoenmak, Lalida ^{[6
]}

Tangboriboonrat, Pramuan ^{[6
]}

机构：

[1] Mahidol Univ, Fac Med Technol, Dept Clin Microbiol, Bangkok 10700, Thailand

[2] Natl Nanotechnol Ctr, Pathum Thani 12120, Thailand

[3] Mahidol Univ, Fac Med Technol, Dept Clin Microscopy, Bangkok 10700, Thailand

[4] Mahidol Univ, Fac Med, Ramathibodi Hosp, Dept Pediat, Bangkok 10700, Thailand

[5] Ramathibodi Hosp, Fac Med, Dept Med, Bangkok 10700, Thailand

[6] Mahidol Univ, Fac Sci, Dept Chem, Bangkok 10400, Thailand

来源：

ANALYST | 2011年 / 136卷 / 02期

关键词：

REAL-TIME PCR; ABL; HYBRIDIZATION; SENSITIVITY; CULTURE;

D O I：

10.1039/c0an00374c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel tool for the detection of BCR/ABL fusion gene in chronic myelogenous leukemia (CML) was developed by a magneto-polymerase chain reaction (PCR)-enzyme linked gene technique. The forward primers covalently bound to the surface of magnetic nanoparticles allowed a convenient separation of PCR products with high sensitivity (0.5 pg ml(-1)) and high specificity using K562 cell line and CML patients. The results were obtained when the biotinylated-reverse primer bound to streptavidin-horseradish peroxidase (HRP) and hydrolysed the substrate. This novel readout system was approximately 1000-fold more sensitive than the conventional agarose gel electrophoresis. The present technique is practical and useful for following up CML patients and for providing appropriate treatment, particularly to patients in remote areas.

引用

页码：354 / 358

页数：5

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