Angiotensin II-mediated suppression of synaptic proteins in mouse hippocampal neuronal HT22 cell was inhibited by propofol: role of calcium signaling pathway

被引：5
作者：
Ding, Xiaowei [1 ]
Ju, Xingzhu [2 ]
Lu, Yan [1 ]
Chen, Wei [1 ]
Wang, Jiaqiang [1 ]
Miao, Changhong [1 ]
Chen, Jiawei [1 ]
机构：
[1] Fudan Univ, Shanghai Canc Ctr, Shanghai Med Coll, Dept Anesthesiol,Dept Oncol, 270 DongAn Rd, Shanghai 200032, Peoples R China
[2] Fudan Univ, Shanghai Canc Ctr, Shanghai Med Coll, Dept Oncol,Dept Gynecol Oncol, 270 DongAn Rd, Shanghai 200032, Peoples R China
来源：
JOURNAL OF ANESTHESIA | 2018年 / 32卷 / 06期
关键词：
Angiotensin II; Hippocampus; Propofol; PSD95; Synapsin I; OXIDATIVE STRESS; CEREBRAL-ISCHEMIA; ALZHEIMERS-DISEASE; INDUCED APOPTOSIS; ACTIVATION; BRAIN; RECEPTOR; PROTECTS; DEATH; BETA;
D O I：
10.1007/s00540-018-2565-x
中图分类号：
R614 [麻醉学];
学科分类号：
100217 ;
摘要：
PurposeAngiotensin II (Ang II) has been shown to be involved in neurological disorders. Propofol demonstrated neuroprotective effects in neurons.MethodsMouse hippocampal HT22 cells were pre-treated with propofol, followed by Ang II treatment. The expression of synaptic proteins (synapsin I and PSD95) was examined. The effects of propofol on Ang II-induced NADPH oxidase expression and superoxide anion generation were examined. The effects of propofol on intracellular calcium concentration, the activation of calcium/calmodulin-dependent protein kinase II (CaMKII), and protein kinase C (PKC) were measured.ResultsAng II reduced the expression of synapsin I and PSD95, which was attenuated by propofol. Ang II-induced effects were blocked by Ang II type 1 receptor (AT1 receptor) blocker. Ang II induced the expression of NADPH oxidase and caused superoxide anion accumulation, which were attenuated by propofol. In addition, propofol induced intracellular calcium concentration, and activated CaMKII as well as PKC. Importantly, the Ang II-mediated effects were diminished by -tocopherol, and the propofol-mediated effects were alleviated by calcium chelator, CaMKII inhibitor, and PKC inhibitor.ConclusionAng II, via AT1 receptor, induced oxidative stress and reduced the expression of synapsin I and PSD95 in HT22 cells. Propofol may increase synapsin I and PSD95 expression by inhibiting oxidative stress and stimulating calcium signaling pathway.
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页码：856 / 865
页数：10
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