Properties of Partially Purified Endopolyphosphatase of the Yeast Saccharomyces cerevisiae

被引：9

作者：

Lichko, L. P. ^{[1
]}

Kulakovskaya, T. V. ^{[1
]}

Kulaev, I. S. ^{[1
]}

机构：

[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Region, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 11期

基金：

俄罗斯基础研究基金会;

关键词：

inorganic polyphosphates; endopolyphosphatase; exopolyphosphatase; cytosol; PPX1 and PPN1 mutants; Saccharomyces cerevisiae; MOLECULAR-MASS EXOPOLYPHOSPHATASE; INORGANIC POLYPHOSPHATE; PPN1; GENES; INACTIVATION; PPX1; DEPENDENCE; CYTOSOL; GROWTH;

D O I：

10.1134/S0006297910110131

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Partially purified endopolyphosphatase from cytosol of the yeast Saccharomyces cerevisiae with inactivated genes PPX1 and PPN1 encoding exopolyphosphatases was obtained with ion_exchange and affinity chromatography. The enzyme activity was estimated by decrease of polyphosphate chain length determined by PAGE. The enzyme cleaved inorganic polyphosphate without the release of orthophosphate (P(i)) and was inhibited by heparin and insensitive to fluoride. Mg(2+), Mn(2+), and Co(2+) (1.5 mM) stimulated the activity, and Ca(2+) was ineffective. The molecular mass of the endopolyphosphatase determined by gel filtration was of a parts per thousand 20 kDa.

引用

页码：1404 / 1407

页数：4

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