Acetylated cashew gum and fucan for incorporation of lycopene rich extract from red guava (Psidium guajava']java L.) in nanostructured systems: Antioxidant and antitumor capacity

Industrial application of lycopene is limited due to its chemical instability and low bioavailability. This study proposes the development of fucan-coated acetylated cashew gum nanoparticles (NFGa) and acetylated cashew gum nanoparticles (NGa) for incorporation of the lycopene-rich extract from red guava (LEG). Size, poly-dispersity, zeta potential, nanoparticles concentration, encapsulation efficiency, transmission electron micro-scopy (TEM) and atomic force microscopy (AFM) were used to characterize nanoparticles. The antioxidant activity was determinated and cell viability was evaluated in the human breast cancer cells (MCF-7) and human keratinocytes (HaCaT) by MTT assay. The toxic effect was evaluated by hemolysis test and by Galleria mellonella model. NFGa showed higher stability than NGa, having a size of 162.10 +/- 3.21 nm, polydispersity of 0.348 +/- 0.019, zeta potential-30.70 +/- 0.53 mV, concentration of 6.4 x 10(9) nanoparticles/mL and 60% LEG encapsu-lation. Microscopic analysis revealed a spherical and smooth shape of NFGa. NFGa showed antioxidant capacity by ABTS method and ORAC assay. The NFGa presented significant cytotoxicity against MCF-7 from the lowest concentration tested (6.25-200 mu g/mL) and did not affect the cell viability of the HaCaT. NFGa showed non-toxic effect in the in vitro and in vivo models. Therefore, NFGa may have a promising application in LEG stabilization for antioxidant and antitumor purposes.