Bishydrazide Glycoconjugates for Lectin Recognition and Capture of Bacterial Pathogens

被引：26

作者：

Adak, Avijit Kumar ^{[1
]}

Leonov, Alexei P. ^{[1
]}

Ding, Ning ^{[1
]}

Thundimadathil, Jyothi ^{[1
]}

Kularatne, Sumith ^{[1
]}

Low, Philip S. ^{[1
]}

Wei, Alexander ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

BIOCONJUGATE CHEMISTRY | 2010年 / 21卷 / 11期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

SELF-ASSEMBLED MONOLAYERS; PSEUDOMONAS-AERUGINOSA; CARBOHYDRATE MICROARRAYS; GOLD SURFACES; ONE-STEP; GLYCANS; IMMOBILIZATION; NANOPARTICLES; FABRICATION; BINDING;

D O I：

10.1021/bc100288c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bishydrazides are versatile linkers for attaching glycans to substrates for lectin binding and pathogen detection schemes. The alpha,omega-bishydrazides of carboxymethylated hexa(ethylene glycol) (4) can be conjugated at one end to unprotected oligosaccharides, then attached onto carrier proteins, tethered onto activated carboxyl-terminated surfaces, or functionalized with a photoactive cross-linking agent for lithographic patterning. Glycoconjugates of bishydrazide 4 can also be converted into dithiocarbamates (DTCs) by treatment with CS2 under mild conditions, for attachment onto gold substrates. The immobilized glycans serve as recognition elements for cell-surface lectins and enable the detection and capture of bacterial pathogens such as Pseudomonas aeruginosa by their adsorption onto micropatterned substrates. A detection limit of 10(3) cfu/mL is demonstrated, using a recently introduced method based on optical pattern recognition.

引用

页码：2065 / 2075

页数：11

共 91 条

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