FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells

被引：49
作者：
Farre, Jordi
Roura, Santiago
Prat-Vidal, Cristina
Soler-Botija, Carolina
Llach, Anna
Molina, Cristina E.
Leifhove-Madsen
Cairo, Jordi J.
Godia, Francesc
Bragos, Ramon
Cinca, Juan
Bayes-Genis, Antoni
机构：
[1] Hosp Santa Creu & Sant Pau, Dept Cardiol, Cardiac Regenrat Program, E-08025 Barcelona, Spain
[2] Univ Autonoma Barcelona, Hosp Santa Creu & Sant Pau, Inst Catala Ciencies Cardiovasc, Dept Cardiol,Lab Cell Physiol, E-08025 Barcelona, Spain
[3] Univ Autonoma Barcelona, Dept Engn Quim, Escola Tecn Super Engn, ETSE, E-08193 Barcelona, Spain
[4] Univ Politecn Cataluna, Ctr Recerca Engn Biomed, ES-08034 Barcelona, Spain
来源：
GROWTH FACTORS | 2007年 / 25卷 / 02期
关键词：
mesenchymal stem cells; FGF-4; growth; pluripotency; cardiomyogenic;
D O I：
10.1080/08977190701345200
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 +/- 0.2 vs. 4.1 +/- 0.2 days, respectively; p=0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.
引用
收藏
页码：71 / 76
页数：6
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