Human papillomavirus gene expression in cutaneous squamous cell carcinomas from immunosuppressed and immunocompetent individuals

被引:66
作者
Purdie, KJ
Surentheran, T
Sterling, JC
Bell, L
McGregor, JM
Proby, CM
Harwood, CA
Breuer, J
机构
[1] Queen Mary Univ London, Barts & London Sch Med & Dent, Ctr Cutaneous Res, London E1 2AT, England
[2] Queen Mary Univ London, Barts & London Sch Med & Dent, Skin Tumour Lab, Canc Res UK, London E1 2AT, England
[3] Queen Mary Univ London, Barts & London Sch Med & Dent, Skin Virus Lab, London E1 2AT, England
[4] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England
关键词
human papillomavirus; immunosuppression; organ transplantation; squamous cell carcinoma;
D O I
10.1111/j.0022-202X.2005.23635.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Epidermodysplasia verruciformis (EV)-type human papillomavirus (HPV) DNA have been detected by PCR in squamous cell carcinomas (SCC) from both organ transplant recipients (OTR) and immunocompetent individuals. Their role in skin cancer remains unclear, and previous studies have not addressed whether the viruses are transcriptionally active. We have used in situ hybridization to investigate the transcriptional activity and DNA localization of HPV. EV-HPV gene transcripts were demonstrated in four of 11 (36%) OTR SCC, one of two (50%) IC SCC, and one of five (20%) OTR warts positive by PCR. Viral DNA co-localized with E2/E4 early region gene transcripts in the middle or upper epidermal layers. Non-EV cutaneous HPV gene transcripts were demonstrated in one of five (20%) OTR SCC and four of 10 (40%) OTR warts. In mixed infections transcripts for both types were detected in two of six (33%) cases. Our results provide evidence of EV-HPV gene expression in SCC; although only a proportion of tumors were positive, the similarly low transcriptional activity in warts suggests this is an underestimate. These observations, together with emerging epidemiological and functional data, provide further reason to focus on the contribution of EV-HPV types to the pathogenesis of cutaneous SCC.
引用
收藏
页码:98 / 107
页数:10
相关论文
共 38 条
  • [1] The ubiquity and impressive genomic diversity of human skin papillomaviruses suggest a commensalic nature of these viruses
    Antonsson, A
    Forslund, O
    Ekberg, H
    Sterner, G
    Hansson, BG
    [J]. JOURNAL OF VIROLOGY, 2000, 74 (24) : 11636 - 11641
  • [2] The presence of antibodies against virus-like particles of epidermodysplasia verruciformis-associated human papillomavirus type 8 in patients with actinic keratoses
    Bavinck, JNB
    Stark, S
    Petridis, AK
    Marugg, ME
    Ter Schegget, J
    Westendorp, RGJ
    Fuchs, PG
    Vermeer, BJ
    Pfister, H
    [J]. BRITISH JOURNAL OF DERMATOLOGY, 2000, 142 (01) : 103 - 109
  • [3] THE PREDOMINANT MESSENGER-RNA CLASS IN HPV16-INFECTED GENITAL NEOPLASIAS DOES NOT ENCODE THE E6 OR THE E7 PROTEIN
    BOHM, S
    WILCZYNSKI, SP
    PFISTER, H
    IFTNER, T
    [J]. INTERNATIONAL JOURNAL OF CANCER, 1993, 55 (05) : 791 - 798
  • [4] The causal relation between human papillomavirus and cervical cancer
    Bosch, FX
    Lorincz, A
    Muñoz, N
    Meijer, CJLM
    Shah, KV
    [J]. JOURNAL OF CLINICAL PATHOLOGY, 2002, 55 (04) : 244 - 265
  • [5] Boxman ILA, 2000, INT J CANCER, V86, P118, DOI 10.1002/(SICI)1097-0215(20000401)86:1&lt
  • [6] 118::AID-IJC18&gt
  • [7] 3.0.CO
  • [8] 2-2
  • [9] Association between epidermodysplasia verruciformis- associated human papillomavirus DNA in plucked eyebrow hair and solar keratoses
    Boxman, ILA
    Russell, A
    Mulder, LHC
    Bavinck, JNB
    ter Schegget, J
    Green, A
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (05) : 1108 - 1112
  • [10] Detection of human papillomavirus DNA in plucked hairs from renal transplant recipients and healthy volunteers
    Boxman, ILA
    Berkhout, RJM
    Mulder, LHC
    Wolkers, MC
    Bavinck, JNB
    Vermeer, BJ
    terSchegget, J
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1997, 108 (05) : 712 - 715