Human mesenchymal stem cells promote growth of osteosarcoma: Involvement of interleukin-6 in the interaction between human mesenchymal stem cells and Saos-2

被引：76
作者：
Bian, Zhen-Yu [1 ,2 ]
Fan, Qi-Ming [1 ]
Li, Gang [3 ]
Xu, Wen-Ting [1 ]
Tang, Ting-Ting [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Shanghai Key Lab Orthopaed Implant, Dept Orthopaed Surg, Sch Med,Shanghai Peoples Hosp 9, Shanghai 200030, Peoples R China
[2] Hangzhou First Peoples Hosp, Dept Orthopaed Surg, Hangzhou, Zhejiang, Peoples R China
[3] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Hong Kong, Hong Kong, Peoples R China
来源：
CANCER SCIENCE | 2010年 / 101卷 / 12期
关键词：
WNT GENE FAMILY; BONE-MARROW; TUMOR STROMA; PROGENITOR CELLS; IL-6; FUNCTIONS; IN-VITRO; CYTOKINES; DELIVERY; VEHICLES; MODEL;
D O I：
10.1111/j.1349-7006.2010.01731.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Our previous study showed that exogenous human mesenchymal stem cells (hMSCs) targeted established osteosarcoma and promoted its growth and pulmonary metastasis in vivo. As a follow-up, the present study aimed to investigate how hMSCs would interact with Saos-2 through autocrine/paracrine communication. The results showed that co-injection of hMSCs with Saos-2 into the proximal tibia of nude mice could promote tumor growth and progression. In vitro, the proliferation of Saos-2 and hMSCs was promoted by each other's conditioned medium, in which interleukin-6 (IL-6) played an important role. Osteogenic differentiation of hMSCs could be inhibited by conditioned medium of Saos-2, in which IL-6 was also involved. Furthermore, decreased IL-6 secretion by hMSCs during its osteogenesis and increased IL-6 secretion in response to conditioned medium of Saos-2 were observed. Based on these data, we suggest that there was a positive feedback loop of IL-6 in the interaction between hMSCs and Saos-2. (Cancer Sci 2010; 101: 2554-2560).
引用
收藏
页码：2554 / 2560
页数：7
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