Culture Condition Improvement for Phytase Production in Solid State Fermentation by Aspergillus ficuum Using Statistical Method

In this study, the effective factors on phytase production by Aspergillus ficuum PTCC 5288 was investigated using solid-state fermentation method in 250 ml shake flask. The process parameters influencing phytase production were identified using Plackett-Burman design. Among the various variables screened, four factors including glucose, moisture, MgSO4 and fermentation time were most significant. The optimum levels of these significant parameters were determined via response surface methodological approach as: 10.14 % glucose, 62.69 % moisture, 0.46 % MgSO4 and 119.23 h. The maximum predicted amount of phytase was 24.33 U/gds and the produced amount of phytase under these conditions was 25.6 U/gds, which indicates the efficacy of the model for prediction of phytase production content under different conditions of the medium.