Differential Scanning Calorimetry Study on Thermal Denaturation of Human Carbonic Anhydrase II

被引：18

作者：

Amani, Mojtaba ^{[1
]}

Khodarahmi, Reza ^{[2
,3
]}

Ghobadi, Sirous ^{[4
]}

Mehrabi, Masomeh ^{[2
,3
,4
]}

Kurganov, Boris I. ^{[5
]}

Moosavi-Movahedi, Ali Akbar ^{[6
]}

机构：

[1] Ardabil Univ Med Sci, Fac Med, Ardebil, Iran

[2] Kermanshah Univ Med Sci, Dept Pharmacognosy & Biotechnol, Fac Pharm, Kermanshah, Iran

[3] Kermanshah Univ Med Sci, Med Biol Res Ctr, Kermanshah, Iran

[4] Razi Univ, Dept Biol, Fac Sci, Kermanshah, Iran

[5] Russian Acad Sci, AN Bakh Biochem Inst, Moscow, Russia

[6] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran

来源：

JOURNAL OF CHEMICAL AND ENGINEERING DATA | 2011年 / 56卷 / 04期

基金：

美国国家科学基金会;

关键词：

AGGREGATION; STABILITY; BETA;

D O I：

10.1021/je101087j

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

The thermal unfolding of human carbonic anhydrase II (HCAII) has been studied by circular dichroism, UV-vis spectrophotometry, and differential scanning calorimetry (DSC). Coincidence of aggregation and tertiary structure disruption as well as fitting of DSC data showed that a two-state model can properly explain thermal unfolding of HCAII. According to this model, the average values of T* (the temperature at which k = 1/60 s(-1)), Delta H (enthalpy), and Delta E-a (activation energy) are equal to 335.8 K, 698.6 kJ.mol(-1), and 529.0 kJ.mol(-1), respectively.

引用

页码：1158 / 1162

页数：5

共 22 条

[1] Unfolding a folding disease:: Folding, misfolding and aggregation of the marble brain syndrome-associated mutant H107Y of human carbonic anhydrase II [J].