Inhibition of the DevSR Two-Component System by Overexpression of Mycobacterium tuberculosis PknB in Mycobacterium smegmatis

被引：14

作者：

Bae, Hyun-Jung ^{[1
]}

Lee, Ha-Na ^{[1
]}

Baek, Mi-Na ^{[1
]}

Park, Eun-Jin ^{[1
]}

Eom, Chi-Yong ^{[2
]}

Ko, In-Jeong ^{[3
]}

Kang, Ho-Young ^{[1
]}

Oh, Jeong-Il ^{[1
]}

机构：

[1] Pusan Natl Univ, Dept Microbiol, Busan 46241, South Korea

[2] Korea Basic Sci Inst, Seoul 02855, South Korea

[3] Korea Adv Inst Sci & Technol, Korea Sci Acad, Busan 47162, South Korea

来源：

MOLECULES AND CELLS | 2017年 / 40卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

DevSR; mycobacterium; Ser/Thr protein kinase; two-component system; RESPONSE REGULATOR; HYPOXIC RESPONSE; PROTEIN-KINASES; SERINE/THREONINE KINASES; SIGNALING PATHWAYS; HISTIDINE KINASES; SER/THR; SENSOR; PHOSPHORYLATION; MECHANISM;

D O I：

10.14348/molcells.2017.0076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DevSR (DosSR) two-component system, which is a major regulatory system involved in oxygen sensing in mycobacteria, plays an important role in hypoxic induction of many genes in mycobacteria. We demonstrated that overexpression of the kinase domain of Mycobacterium tuberculosis (Mtb) PknB inhibited transcriptional activity of the DevR response regulator in Mycobacterium smegmatis and that this inhibitory effect was exerted through phosphorylation of DevR on Thr180 within its DNA-binding domain. Moreover, the purified kinase domain of Mtb PknB significantly phosphorylated RegX3, NarL, KdpE, TrcR, DosR, and MtrA response regulators of Mtb that contain the Thr residues corresponding to Thr180 of DevR in their DNA-binding domains, implying that transcriptional activities of these response regulators might also be inhibited when the kinase domain of PknB is overexpressed.

引用

页码：632 / 642

页数：11

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