Freeze-dried Saccharomyces cerevisiae cells immobilized on gluten pellets for glucose fermentation

被引:37
作者
Bekatorou, A [1 ]
Koutinas, AA [1 ]
Kaliafas, A [1 ]
Kanellaki, M [1 ]
机构
[1] Univ Patras, Sect Analyt Environm & Appl Chem, Dept Chem, Patras 26500, Greece
关键词
fermentation; freeze-dried bakers' yeast cells; immobilized yeast cells; gluten pellets;
D O I
10.1016/S0032-9592(00)00246-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
No protecting medium was used for the freeze-drying of Saccharomyces cerevisiae free cells and those immobilized on gluten pellets to form an immobilized biocatalyst. The use of these for glucose fermentations at various initial glucose concentrations, temperatures and initial pH values showed that the fermentation time for the first batch with freeze-dried immobilized cells was 1.5- to 3-fold less than that with free freeze-dried cells. In the second repeated batch, the fermentation time of freeze-dried immobilized cells was less, in some cases up to three times less than the first batch and reached the fermentation time of fresh immobilized cells. After storage for 16 months at 4 degreesC, freeze-dried immobilized biocatalyst was used for five repeated batch fermentations of glucose. The fermentation time decreased continuously until 18 h, which was less than the half of the fermentation time of the first batch. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:549 / 557
页数:9
相关论文
共 18 条
[1]   Room and low temperature brewing with yeast immobilized on gluten pellets [J].
Bardi, E ;
Koutinas, AA ;
Kanellaki, M .
PROCESS BIOCHEMISTRY, 1997, 32 (08) :691-696
[2]   Room temperature and low temperature wine making using yeast immobilized on gluten pellets [J].
Bardi, EP ;
Bakoyianis, V ;
Koutinas, AA ;
Kanellaki, M .
PROCESS BIOCHEMISTRY, 1996, 31 (05) :425-430
[3]   Immobilization of yeast on delignified cellulosic material for low temperature brewing [J].
Bardi, EP ;
Koutinas, AA ;
Soupioni, MJ ;
Kanellaki, ME .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (02) :463-467
[4]   IMMOBILIZATION OF YEAST ON DELIGNIFIED CELLULOSIC MATERIAL FOR ROOM-TEMPERATURE AND LOW-TEMPERATURE WINE-MAKING [J].
BARDI, EP ;
KOUTINAS, AA .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1994, 42 (01) :221-226
[5]   VIABILITY AND STABILITY OF YEAST-CELLS AND FILAMENTOUS FUNGUS SPORES DURING FREEZE-DRYING - EFFECTS OF PROTECTANTS AND COOLING RATES [J].
BERNY, JF ;
HENNEBERT, GL .
MYCOLOGIA, 1991, 83 (06) :805-815
[6]   FREEZE-DRYING OF MICROORGANISMS - INFLUENCE OF COOLING RATE ON SURVIVAL [J].
GEHRKE, HH ;
PRALLE, K ;
DECKWER, WD .
FOOD BIOTECHNOLOGY, 1992, 6 (01) :35-49
[7]  
KANA K, 1988, J FOOD SCI, V56, P1723
[8]  
KANA K, 1988, J FOOD SCI, V53, P1749
[9]   THE LOSS OF PLASMID-ENCODED PHENOTYPES IN ALCALIGENES-EUTROPHUS, STAPHYLOCOCCUS-AUREUS, AND PSEUDOMONAS-PUTIDA DURING FREEZE-DRYING AND STORAGE [J].
LANGE, CR ;
WEBER, AS .
WATER ENVIRONMENT RESEARCH, 1995, 67 (02) :224-229
[10]   Recent advances in the malting and brewing industry [J].
Linko, M ;
Haikara, A ;
Ritala, A ;
Penttilä, M .
JOURNAL OF BIOTECHNOLOGY, 1998, 65 (2-3) :85-98