Capturing Greater 16S rRNA Gene Sequence Diversity within the Domain Bacteria

被引：43

作者：

Winsley, T. ^{[1
]}

van Dorst, J. M. ^{[1
]}

Brown, M. V. ^{[1
]}

Ferrari, B. C. ^{[1
]}

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 16期

关键词：

MICROBIAL DIVERSITY; PRIMERS; DESIGN;

D O I：

10.1128/AEM.01299-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A large proportion of "universal" 16S PCR primers lack sequence homology to many of the "candidate" divisions, severely limiting bacterial diversity assessments. We designed a primer set that offers a 50% increase in silico in coverage of the domain Bacteria over the commonly used primer combination 27F/519R. Comparisons using pyrosequencing on soil environments showed a significant increase in recovery of taxonomic diversity with around a 3-fold increase in recovery of sequences from candidate divisions.

引用

页码：5938 / 5941

页数：4

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