Using maximum heart rate as a rapid screening tool to determine optimum temperature for aerobic scope in Pacific salmon Oncorhynchus spp.

The mean +/- S.E. optimum temperature (Topt) for aerobic scope in juvenile coho salmon Oncorhynchus kisutch was determined to be 17.0 +/- 0.7 degrees C. The repeated measures protocol took 3 weeks to complete the T-opt determination using 12 fish tested at five temperatures separated by 2 degrees C increments. This experiment also demonstrated that the T-opt was associated with maximum heart rate (f(H)) failing to maintain a Q(10)-related increase with temperature. When maximum f(H) was produced in anaesthetized fish with pharmacological stimulation and f(H) measured from electrocardiogram recordings during acute warming, the Arrhenius break temperature (ABT) for Q(10) discontinuities in maximum f(H) (mean +/- S.E. = 17.1 +/- 0.5 degrees C for 15 ppm clove oil and 16.5 +/- 0.2 degrees C for 50 ppm MS-222) was statistically indistinguishable from the T-opt measured using aerobic scope. Such a determination took only 3 days rather than 3 weeks. Therefore, it is proposed that determining ABT for discontinuities in maximum f(H) in anaesthetized fish presents itself as a valuable, high-throughput screening tool to assess T-opt in fishes, a metric that has become recognized as being extremely valuable in fish biology and fisheries management. (C) 2012 The Authors Journal of Fish Biology (C) 2012 The Fisheries Society of the British Isles