High-throughput screening assay for D-amino acid oxidase

被引：10

作者：

Khoronenkova, Svetlana V. ^{[1
,2
]}

Tishkov, Vladimir I. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem Enzymol, Moscow 119992, Russia

[2] Innovat & High Technol MSU Ltd, Moscow 109551, Russia

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 374卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

D-amino acid oxidase; high-throughput screening; peroxidase reaction; membrane; recombinant Escherichia coli cells; mutant library;

D O I：

10.1016/j.ab.2007.12.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A membrane-based high-throughput screening (HTS) assay for active D-amino acid oxidase (DAAO) in liquid samples as well as in intact Escherichia coli cells has been developed and optimized. The detection limit of the assay was less than 1 ng per sample. The method proposed can be used for quantitative DAAO determination in the range of 0.13 to 3.60 ng enzyme per probe. The protocol was successfully tested to screen a library of E coli clones containing mutant DAAOs active toward target substrates. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：405 / 410

页数：6

共 23 条

[1] Deracemisation and stereoinversion of α-amino acids using D-amino acid oxidase and hydride reducing agents [J].

Beard, TM ;

Turner, NJ .

CHEMICAL COMMUNICATIONS, 2002, (03) :246-247

[2] The discovery of susceptibility genes for mental disorders [J].

Cloninger, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13365-13367

[3] USE OF DIAMINOBENZIDINE FOR SPECTROPHOTOMETRIC AND ACRYLAMIDE-GEL DETECTION OF SULFITE OXIDASE AND ITS APPLICABILITY TO HYDROGEN PEROXIDE-GENERATING ENZYMES [J].

COHEN, HJ .

ANALYTICAL BIOCHEMISTRY, 1973, 53 (01) :208-222

[4] 2-STEP IMMOBILIZED ENZYME CONVERSION OF CEPHALOSPORIN-C TO 7-AMINOCEPHALOSPORANIC ACID [J].

CONLON, HD ;

BAQAI, J ;

BAKER, K ;

SHEN, YQ ;

WONG, BL ;

NOILES, R ;

RAUSCH, CW .

BIOTECHNOLOGY AND BIOENGINEERING, 1995, 46 (06) :510-513

[5]

Davydova E. E, 2002, MOSC U CHEM B, V43, P353

[6] Chiral analysis of amino acids using electrochemical composite bienzyme biosensors [J].

Domínguez, R ;

Serra, B ;

Reviejo, AJ ;

Pingarrón, JM .

ANALYTICAL BIOCHEMISTRY, 2001, 298 (02) :275-282

[7] Detection and substrate selectivity of new microbial D-amino acid oxidases [J].

Gabler, M ;

Hensel, M ;

Fischer, L .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (08) :605-611

[8] Genes for schizophrenia? Recent findings and their pathophysiological implications [J].

Harrison, PJ ;

Owen, MJ .

LANCET, 2003, 361 (9355) :417-419

[9] Development of a D-alanine sensor for the monitoring of a fermentation using the improved selectivity by the combination of D-amino acid oxidase and pyruvate oxidase [J].

Inaba, Y ;

Mizukarni, K ;

Hamada-Sato, N ;

Kobayashi, T ;

Imada, C ;

Watanabe, E .

BIOSENSORS & BIOELECTRONICS, 2003, 19 (05) :423-431

[10] Substitution of the critical methionine residues in Trigonopsis variabilis D-amino acid oxidase with leucine enhances its resistance to hydrogen peroxide [J].

Ju, SS ;

Lin, LL ;

Chien, HCR ;

Hsu, WH .

FEMS MICROBIOLOGY LETTERS, 2000, 186 (02) :215-219

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