Transcriptome Analysis of Gills Provides Insights Into Translation Changes Under Hypoxic Stress and Reoxygenation in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

被引:20
作者
San, Lize [1 ,2 ]
Liu, Baosuo [1 ,3 ,4 ]
Liu, Bo [1 ,3 ]
Guo, Huayang [1 ,3 ,5 ]
Guo, Liang [1 ,3 ]
Zhang, Nan [1 ,3 ]
Zhu, Kecheng [1 ,3 ,4 ,5 ]
Jiang, Shigui [1 ,3 ]
Zhang, Dianchang [1 ,3 ,4 ,5 ]
机构
[1] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Peoples R China
[2] Hebei Agr Univ, Ocean Coll, Qinhuangdao, Hebei, Peoples R China
[3] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Trop Aquaculture Res & Dev Ctr, Sanya, Peoples R China
[4] Guangdong Prov Engineer Technol Res Ctr Marine Bi, Guangzhou, Peoples R China
[5] Sanya Trop Fisheries Res Inst, Sanya, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
Trachinotus ovatus; hypoxia stress; transcriptome; gene expression; gills; ANTIOXIDANT DEFENSE; CRUCIAN CARP; RESPONSES; CONSEQUENCES; STRATEGIES; CROCEA;
D O I
10.3389/fmars.2021.763622
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Golden pompano (Trachinotus ovatus) is one of the most economically critical marine fish in South China. Low oxygen stress has resulted in substantial economic losses to the aquaculture of T. ovatus. However, the molecular responses of fish gills to hypoxia challenge remain unclear. To understand the mechanism underlying adaption to hypoxia, we analyzed the transcriptome of T. ovatus gills in response to hypoxic stress in the normal oxygen group, hypoxic group, and hypoxia treatment after oxygen recovery group. This study found that hypoxia for 8 h was the critical time of hypoxic stress and corresponded to the largest number of differentially expressed genes. After hypoxic stress, genes for chemokines, chemokine receptors, interleukins, complement factors, and other cytokines were significantly downregulated, which may be why fish are vulnerable to pathogen infection in a hypoxic environment. According to a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, many downregulated genes were significantly enriched in the steroid biosynthesis, focal adhesion, and the extracellular matrix (ECM)-receptor interaction signal pathways, which affected cell signal transduction, adhesion, and apoptosis. Compared with the hypoxic group, the amounts of upregulated genes related to phagocytosis and protein degradation were upregulated in the dissolved oxygen recovery group. These results indicated that after the recovery of dissolved oxygen, the fish body repaired the stress-induced damage by rapidly removing misfolded proteins. These findings provide a better understanding of the hypoxia response mechanism of fish and represent a useful resource for the genetic breeding of T. ovatus.
引用
收藏
页数:14
相关论文
共 44 条
[1]   The integument of the nonamphibious goby Gobionellus oceanicus: Its functional morphology and respiratory capacity [J].
Aguilar, Leticia ;
Leite, Rebeca Nuno ;
Ferreira, Cleomarcia Almeida ;
da Cruz, Andre Luis .
JOURNAL OF MORPHOLOGY, 2018, 279 (11) :1548-1558
[2]   Talins and kindlins: partners in integrin-mediated adhesion [J].
Calderwood, David A. ;
Campbell, Iain D. ;
Critchley, David R. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2013, 14 (08) :503-517
[3]   Metabolic and cardiorespiratory responses of summer flounder Paralichthys dentatus to hypoxia at two temperatures [J].
Capossela, K. M. ;
Brill, R. W. ;
Fabrizio, M. C. ;
Bushnell, P. G. .
JOURNAL OF FISH BIOLOGY, 2012, 81 (03) :1043-1058
[4]   fastp: an ultra-fast all-in-one FASTQ preprocessor [J].
Chen, Shifu ;
Zhou, Yanqing ;
Chen, Yaru ;
Gu, Jia .
BIOINFORMATICS, 2018, 34 (17) :884-890
[5]   Transcriptome and physiology analysis identify key metabolic changes in the liver of the large yellow croaker (Larimichthys crocea) in response to acute hypoxia [J].
Ding, Jie ;
Liu, Cheng ;
Luo, Shengyu ;
Zhang, Yibo ;
Gao, Xinming ;
Wu, Xiongfei ;
Shen, Weiliang ;
Zhu, Junquan .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2020, 189
[6]   Transcriptomic Profiling of the Adaptive and Innate Immune Responses of Atlantic Salmon to Renibacterium salmoninarum Infection [J].
Eslamloo, Khalil ;
Caballero-Solares, Albert ;
Inkpen, Sabrina M. ;
Emam, Mohamed ;
Kumar, Surendra ;
Bouniot, Camila ;
Avendano-Herrera, Ruben ;
Jakob, Eva ;
Rise, Matthew L. .
FRONTIERS IN IMMUNOLOGY, 2020, 11
[7]   Clinical spectrum and genetic variations of LMNA-related muscular dystrophies in a large cohort of Chinese patients [J].
Fan, Yanbin ;
Tan, Dandan ;
Song, Danyu ;
Zhang, Xu ;
Chang, Xingzhi ;
Wang, Zhaoxia ;
Zhang, Cheng ;
Chan, Sophelia Hoi-Shan ;
Wu, Qixi ;
Wu, Liwen ;
Wang, Shuang ;
Yan, Hui ;
Ge, Lin ;
Yang, Haipo ;
Mao, Bing ;
Boennemann, Carsten ;
Liu, Jingying ;
Wang, Suxia ;
Yuan, Yun ;
Wu, Xiru ;
Zhang, Hong ;
Xiong, Hui .
JOURNAL OF MEDICAL GENETICS, 2021, 58 (05) :326-333
[8]   FINE ANATOMICAL STRUCTURES OF THE INTESTINE IN RELATION TO RESPIRATORY FUNCTION OF AN AIR-BREATHING LOACH, LEPIDOCEPHALICHTHYS GUNTEA (ACTINOPTERYGII: CYPRINIFORMES: COBITIDAE) [J].
Ghosh, Saroj K. ;
Ghosh, Bidisha ;
Chakrabarti, Padmanabha .
ACTA ICHTHYOLOGICA ET PISCATORIA, 2011, 41 (01) :1-5
[9]  
Ibanez LG, 2014, IMMUNOLOGY, V143, P183
[10]   A relationship between metabolic rate and risk-taking behaviour is revealed during hypoxia in juvenile European sea bass [J].
Killen, Shaun S. ;
Marras, Stefano ;
Ryan, Mary R. ;
Domenici, Paolo ;
McKenzie, David J. .
FUNCTIONAL ECOLOGY, 2012, 26 (01) :134-143