Geraniin inhibits bladder cancer cell growth via regulation of PI3K/AKT signaling pathways

被引：3

作者：

Xu, Junwei ^{[1
]}

Qin, Ning ^{[2
]}

Yao, Yebin ^{[3
]}

Chen, Tao ^{[1
]}

Jiang, Wenbo ^{[4
]}

机构：

[1] Nantong Univ, Affiliated Hosp, Dept Tradit Chinese Med, Nantong City 226001, Jiangsu, Peoples R China

[2] Nantong Hlth Coll Jiangsu Prov, Dept Med Nursing Teaching & Res, Nantong City 226001, Jiangsu, Peoples R China

[3] Nantong Univ, Affiliated Hosp, Dept Infect Dis, Nantong City 226001, Jiangsu, Peoples R China

[4] Sudian Hosp Tradit Chinese Med, Dept Cardiovasc Med, Suqian City 223800, Jiangsu, Peoples R China

来源：

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH | 2020年 / 19卷 / 02期

关键词：

Geraniin; T24; cells; Apoptosis; PI3K/AKT signaling; SUPPRESSES; APOPTOSIS; MIGRATION; INVASION;

D O I：

10.4314/tjpr.v19i2.6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Purpose: The effect of geraniin on human bladder transitional carcinoma was not clear, this study was thus intended to reveal it and reveal the mechanism. Methods: T24 cells were treated with 5, 10, and 20 mu M of geraniin and the viability and apoptosis of T24 cells were determined using thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometry. The protein expression levels of Cyclin D1, p21, BAL-2, BAX, cleaved caspase-3 and PI3K/AKT pathway were evaluated using western blot. Results: Geraniin decreased T24 cell viability and induced T24 cell cycle arrest. The proportion of T24 cells in S phase was decreased by geraniin. Besides, geraniin promoted T24 cell apoptosis and regulated PI3K/AKT pathway. Conclusion: Geraniin appears to regulate bladder cancer cell growth by decreasing the levels of PI3K and AKT phosphotylation. Thus, this agent may be useful in the management of bladder cancer

引用

页码：253 / 257

页数：5

共 21 条

[1] Digiacomo Graziana., 2018, J. Mol. Clin. Med., V1, P151, DOI [10.31083/j.jmcm.2018.03.004, DOI 10.31083/J.JMCM.2018.03.004]
[2] Bergenin suppresses the growth of colorectal cancer cells by inhibiting PI3K/AKT/mTOR signaling pathway
Gao, Xucan
Wang, Yazhu
Zhang, Jianzhuo
Lin, Lifen
Yao, Qi
Xiang, Guoan
[J]. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, 2017, 16 (10) : 2307 - 2313
[3] Geraniin selectively promotes cytostasis and apoptosis in human colorectal cancer cells by inducing catastrophic chromosomal instability
Guo, Xihan
Wang, Han
Ni, Juan
Liang, Ziqing
Wu, Xiayu
Xue, Jinglun
Wang, Xu
[J]. MUTAGENESIS, 2018, 33 (04) : 271 - 281
[4] SUPERFICIAL BLADDER-CANCER - PROGRESSION AND RECURRENCE
HENEY, NM
AHMED, S
FLANAGAN, MJ
FRABLE, W
CORDER, MP
HAFERMANN, MD
HAWKINS, IR
[J]. JOURNAL OF UROLOGY, 1983, 130 (06) : 1083 - 1086
[5] Exploiting the PI3K/AKT pathway for cancer drug discovery
Hennessy, BT
Smith, DL
Ram, PT
Lu, YL
Mills, GB
[J]. NATURE REVIEWS DRUG DISCOVERY, 2005, 4 (12) : 988 - 1004
[6] Metabolites of the Ellagitannin Geraniin and Their Antioxidant Activities
Ito, Hideyuki
[J]. PLANTA MEDICA, 2011, 77 (11) : 1110 - 1115
[7] Radioprotective effect of geraniin via the inhibition of apoptosis triggered by γ-radiation-induced oxidative stress
Kang, Kyoung Ah
Lee, In Kyung
Zhang, Rui
Piao, Mei Jing
Kim, Ki Cheon
Kim, Sang Young
Shin, Taekyun
Kim, Bum Joon
Lee, Nam Ho
Hyun, Jin Won
[J]. CELL BIOLOGY AND TOXICOLOGY, 2011, 27 (02) : 83 - 94
[8] Bladder cancer
Kaufman, Donald S.
Shipley, William U.
Feldman, Adam S.
[J]. LANCET, 2009, 374 (9685) : 239 - 249
[9] Geraniin inhibits TGF-β1-induced epithelial-mesenchymal transition and suppresses A549 lung cancer migration, invasion and anoikis resistance
Ko, Hyeonseok
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2015, 25 (17) : 3529 - 3534
[10] Antioxidant, anti-semicarbazide-sensitive amine oxidase, and anti-hypertensive activities of geraniin isolated from Phyllanthus urinaria
Lin, Shyr-Yi
Wang, Ching-Chiung
Lu, Yeh-Lin
Wu, Wen-Chun
Hou, Wen-Chi
[J]. FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (07) : 2485 - 2492

← 1 2 3 →