The AlkB Domain of Mammalian ABH8 Catalyzes Hydroxylation of 5-Methoxycarbonylmethyluridine at the Wobble Position of tRNA

被引：125

作者：

Fu, Ye ^{[3
]}

Dai, Qing ^{[1
]}

Zhang, Wen ^{[3
]}

Ren, Jin ^{[3
]}

Pan, Tao ^{[1
,2
]}

He, Chuan ^{[2
,3
]}

机构：

[1] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA

[2] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

[3] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 47期

关键词：

enzyme models; hydroxylation; oxidoreductases; RNA structures; tRNA; OXIDATIVE DEMETHYLATION; DNA-DAMAGE; FTO GENE; ALKYLATION DAMAGE; METHYLTRANSFERASE; CONTRIBUTES; CHILDHOOD; REVERSAL; HOMOLOG; OBESITY;

D O I：

10.1002/anie.201001242

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Family ties: The AlkB family of nonheme iron, α-ketoglutarate (αKG)-dependent dioxygenases is involved in biological processes such as DNA/RNA repair and obesity. The AlkB domain of ABH8 is shown to catalyze the hydroxylation of a modified uridine (mcm5U) into (S)-mchm 5U at the wobble position of the anticodon stem-loop in tRNA (see scheme), thereby suggesting that this protein is a tRNA hypermodification enzyme. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8885 / 8888

页数：4

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