Characterization of a commercial cellulase for hydrolysis of agroindustrial substrates

被引：21

作者：

Balsan, Guilherme ^{[2
]}

Astolfi, Viviane ^{[2
]}

Benazzi, Tassio ^{[2
]}

Meireles, M. Angela A. ^{[3
]}

Maugeri, Francisco ^{[3
]}

Di Luccio, Marco ^{[2
]}

Dal Pra, Valeria ^{[2
]}

Mossi, Altemir J. ^{[4
]}

Treichel, Helen ^{[2
]}

Mazutti, Marcio A. ^{[1
]}

机构：

[1] Univ Fed Santa Maria, Dept Chem Engn, BR-97105900 Santa Maria, RS, Brazil

[2] Univ Reg Integrada Alto Uruguai & Missoes, Dept Food Engn, BR-99700000 Erechim, Brazil

[3] Univ Estadual Campinas, Dept Food Engn, BR-13083862 Campinas, SP, Brazil

[4] Univ Fed Fronteira Sul, Dept Agron, BR-99700000 Erechim, RS, Brazil

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2012年 / 35卷 / 07期

关键词：

Cellulase; Agroindustrial substrates; Enzymatic hydrolysis; Lignocellulosic; THERMAL INACTIVATION; ENZYMATIC-HYDROLYSIS; SOLID-STATE; FERMENTATION; PURIFICATION; ENZYMES; DEACTIVATION; PRETREATMENT; BIOETHANOL; PARAMETERS;

D O I：

10.1007/s00449-012-0710-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to 50 A degrees C and pH from 5.2 to 5.5. Enzyme activity decreased only 15% after 150 h of reaction at temperatures between 30 and 50 A degrees C. No loss of activity was observed at pH 5.0 and 5.5. The cellulase showed satisfactory results in the hydrolysis of agroindustrial substrates, since similar activity was verified on filter paper and other agroindustrial substrates.

引用

页码：1229 / 1237

页数：9

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