Micellar Liquid Chromatographic Method for the Simultaneous Determination of Norfloxacin and Tinidazole in Pharmaceutical Dosage Forms and Human Plasma

被引:0
作者
Walash, M. I. [1 ]
El-Din, M. Sharaf [1 ]
El-Enany, N. [1 ]
Eid, M. [1 ]
Shalan, Sh. [1 ]
机构
[1] Univ Mansoura, Fac Pharm, Dept Analyt Chem, Mansoura 35516, Egypt
来源
LATIN AMERICAN JOURNAL OF PHARMACY | 2011年 / 30卷 / 01期
关键词
Human plasma; Micellar liquid chromatography; Norfloxacin; Pharmaceutical tablets; Tinidazole; FLUORESCENCE DETECTION; DIRECT-INJECTION; URINE; SAMPLES; DIURETICS;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
A micellar liquid chromatographic method was developed for the simultaneous analysis of a binary mixture of norfloxacin and tinidazole (NOR and TIN) in dosage forms and human plasma. The analysis was carried out using a Waters Symmetry (R) C18 column (250 mm x 4.6 mm 5 pm particle size). The running mobile phase consisting of 0.15 M sodium dodecyl sulphate (SDS), 0.3 % triethylamine (TEA), 5 % n-propanol, the pH was adjusted to 4 by addition of 0.02 M orthophosphoric acid pumped at a flow rate 1.0 mL/min with UV at 275 nm. Calibration curves were linear over the range 1-28 and 1.5-42 mu g/mL for NOR and TIN, respectively. The quantification limits were 0.7 and 1.0 mu g /mL for NOR and TIN respectively. The proposed method was successfully applied for the simultaneous determination of NOR and TIN in human plasma without prior precipitation of protein. The mean percentage recoveries of bioavailability test in human plasma (n = 3) were 9031 +/- 4.22 and 90.05 +/- 13 for NOR and TIN, respectively.
引用
收藏
页码:25 / 32
页数:8
相关论文
共 26 条
[1]   Capillary electrophoresis for the determination of norfloxacin and tinidazole in pharmaceuticals with multi-response optimization [J].
Alnajjar, Ahmed ;
AbuSeada, Hamed H. ;
Idris, Abubakr M. .
TALANTA, 2007, 72 (02) :842-846
[2]  
[Anonymous], ICH HARM TRIP GUID V
[3]  
Argekar AP, 1996, ANAL LETT, V29, P1539
[4]  
Argekar AP, 1996, JPC-J PLANAR CHROMAT, V9, P208
[5]   Micellar liquid chromatographic determination of nicotinic acid and nicotinamide after precolumn Konig reaction derivatization [J].
Capella-Peiró, ME ;
Carda-Broch, S ;
Monferrer-Pons, L ;
Esteve-Romero, J .
ANALYTICA CHIMICA ACTA, 2004, 517 (1-2) :81-87
[6]   Direct injection micellar liquid chromatographic method for the analysis of corticosteroids in creams, ointments and other pharmaceuticals [J].
Capella-Peiró, ME ;
Gil-Agustí, M ;
Monferrer-Pons, L ;
Esteve-Romero, J .
ANALYTICA CHIMICA ACTA, 2002, 454 (01) :125-135
[7]   Determination of trazodone in urine and pharmaceuticals using micellar liquid chromatography with fluorescence detection [J].
Carda-Broch, S. ;
Gil-Agusti, M. T. ;
Rambla-Alegre, M. ;
Monferrer-Pons, L. ;
Esteve-Romero, J. S. .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1156 (1-2) :254-258
[8]   Chromatographic determination of diuretics in urine samples using hybrid micellar mobile phases with fluorimetric detection [J].
Carda-Broch, S ;
Esteve-Romero, JS ;
García-Alvarez-Coque, MC .
ANALYTICA CHIMICA ACTA, 1998, 375 (1-2) :143-154
[9]   Micellar liquid chromatographic determination of diuretics by diazotization and coupling with the Bratton-Marshall reagent [J].
CardaBroch, S ;
GarciaAlvarezCoque, MC ;
SimoAlfonso, EF ;
EsteveRomero, JS .
ANALYTICA CHIMICA ACTA, 1997, 353 (2-3) :215-226
[10]  
DAHIBHATE PP, 1997, INDIAN DRUGS, V34, P48