Alleviation of chilling-induced oxidative damage by salicylic acid pretreatment and related gene expression in eggplant seedlings

被引：64
作者：
Chen, Shuangchen [2 ,3 ]
Liu Zimei [2 ]
Cui, Jinxia [2 ]
Ding Jiangang [2 ]
Xia, Xiaojian [2 ]
Liu, Dilin [4 ]
Yu, Jingquan [1 ,2 ]
机构：
[1] Agr Minist China, Key Lab Hort Plants Growth Dev & Biotechnol, Hangzhou 310029, Zhejiang, Peoples R China
[2] Zhejiang Univ, Dept Hort, Hangzhou 310029, Zhejiang, Peoples R China
[3] Henan Univ Sci & Technol, Coll Forestry, Luoyang 471003, Peoples R China
[4] Univ Giessen, Inst Phytopathol & Appl Zool, Giessen, Germany
来源：
PLANT GROWTH REGULATION | 2011年 / 65卷 / 01期
基金：
中国博士后科学基金;
关键词：
Antioxidant system; Chilling stress; Gene expression; Salicylic acid; HYDROGEN-PEROXIDE; STRESS TOLERANCE; GLUTATHIONE; ASCORBATE; ENZYMES; CHLOROPLASTS; APOPLAST; INJURY; LEAVES; PLANTS;
D O I：
10.1007/s10725-011-9579-9
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The effects of salicylic acid (SA) pretreatment on ascorbate-glutathione (GSH) cycle under chilling stress in eggplant seedlings were investigated. Salicylic acid pretreatment improved chilling resistance of eggplant seedlings with maximum efficiency at a concentration of 0.3%. The chilling injury index was decreased by 36.9% as compared to that of the control after 0.3% SA pretreatment. Under chilling stress, seedlings pretreated with SA displayed lower hydrogen peroxide (H2O2) content and higher ascorbate peroxidase (APX) activity than the untreated seedlings. Pretreatment with SA increased ascorbic acid and GSH content. Salicylic acid (0.3%) pretreatment enhanced the dehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) activity but not glutathione reductase (GR). Pretreatment with SA also enhanced the induced expression of stress-responsive genes under chilling stress. The highest transcript levels of GST1, GST2, GPX1, GPX2, GSH, MDHAR, GR and DHAR upon pretreatment with SA (0.3%) were 6.21, 6.24, 7.76, 5.99, 6.54, 6.44, 2.86 and 2.15 folds, respectively as compared to non-SA-treated seedlings. Taken together, SA pre-treatment could effectively protect eggplant seedlings from oxidative damage of chilling stress through enhancing antioxidant enzymes activities and related gene expression.
引用
收藏
页码：101 / 108
页数：8
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