A diverse virome in kidney transplant patients contains multiple viral subtypes with distinct polymorphisms

被引：45

作者：

Rani, Asha ^{[1
]}

Ranjan, Ravi ^{[1
]}

McGee, Halvor S. ^{[1
]}

Metwally, Ahmed ^{[1
,2
]}

Hajjiri, Zahraa ^{[1
]}

Brennan, Daniel C. ^{[3
]}

Finn, Patricia W. ^{[1
]}

Perkins, David L. ^{[1
,2
,4
]}

机构：

[1] Univ Illinois, Dept Med, Chicago, IL 60612 USA

[2] Univ Illinois, Dept Bioengn, Chicago, IL 60612 USA

[3] Washington Univ, Sch Med, Div Renal Dis, St Louis, MO 63110 USA

[4] Univ Illinois, Dept Surg, Chicago, IL 60612 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

BK VIRUS NEPHROPATHY; JC VIRUS; PROTEIN-STRUCTURE; RNA; IDENTIFICATION; REPLICATION; ASSOCIATION; MICROBIOME; EVOLUTION; DIAGNOSIS;

D O I：

10.1038/srep33327

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recent studies have established that the human urine contains a complex microbiome, including a virome about which little is known. Following immunosuppression in kidney transplant patients, BK polyomavirus (BKV) has been shown to induce nephropathy (BKVN), decreasing graft survival. In this study we investigated the urine virome profile of BKV+ and BKV- kidney transplant recipients. Virus-like particles were stained to confirm the presence of VLP in the urine samples. Metagenomic DNA was purified, and the virome profile was analyzed using metagenomic shotgun sequencing. While the BK virus was predominant in the BKV+ group, it was also found in the BKV- group patients. Additional viruses were also detected in all patients, notably including JC virus (JCV) and Torque teno virus (TTV) and interestingly, we detected multiple subtypes of the BKV, JCV and TTV. Analysis of the BKV subtypes showed that nucleotide polymorphisms were detected in the VP1, VP2 and Large T Antigen proteins, suggesting potential functional effects for enhanced pathogenicity. Our results demonstrate a complex urinary virome in kidney transplant patients with multiple viruses with several distinct subtypes warranting further analysis of virus subtypes in immunosuppressed hosts.

引用

页数：13

共 49 条

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