Immobilization of bovine serum albumin on TiO2 film via chemisorption of H3PO4 interface and effects on platelets adhesion

被引：35

作者：

Weng, Y. J. ^{[1
,2
]}

Hou, R. X. ^{[1
,2
]}

Li, G. C. ^{[1
,2
]}

Wang, J. ^{[1
,2
]}

Huang, N. ^{[1
,2
]}

Liu, H. Q. ^{[1
,2
]}

机构：

[1] SW Jiaotong Univ, Minist Educ, Key Lab Adv Technol Mat, Chengdu 610031, Peoples R China

[2] Key Lab Surface Engn Artificial Organs Sichuan, Chengdu 610031, Peoples R China

来源：

APPLIED SURFACE SCIENCE | 2008年 / 254卷 / 09期

关键词：

surface immobilization; TiO2; film; bovine serum albumin; hemocompatibility;

D O I：

10.1016/j.apsusc.2007.10.107

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In the present study, bovine serum albumin (BSA) was successfully covalently immobilized on the surface of anatase TiO2 film by a three-step method, i.e. application of H3PO4 chemisorption to increase surface -OH, which increases the amount of coupling 3-aminopropyl-triethoxylsilane (APTES), thus linking with BSA by imide bond using EDC/NHS/MES. There is no significant -OH group increase on rutile film when using the same method of phosphoric acid treatment, which suggest it is difficult for further chemical modification of the rutile film. After covalent immobilization of BSA on anatase film, an improved hemocompatibility of anti-platelet adhesion and aggregation in vitro could be recognized by LDH and SEM analysis. This study suggests BSA-immobilized anatase surface can serve as hemocompatibility material in vivo. (C) 2007 Elsevier B. V. All rights reserved.

引用

页码：2712 / 2719

页数：8

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