Microfluidic Synthesis of Rigid Nanovesicles for Hydrophilic Reagents Delivery

被引：136

作者：

Zhang, Lu ^{[1
,2
]}

Feng, Qiang ^{[1
,2
]}

Wang, Jiuling ^{[3
]}

Sun, Jiashu ^{[1
,2
]}

Shi, Xinghua ^{[3
]}

Jiang, Xingyu ^{[1
,2
]}

机构：

[1] Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China

[2] Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[3] Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 13期

关键词：

drug delivery; hydrophilic reagents; microfluidics; nanoparticles; vesicles; POLYMER HYBRID NANOPARTICLES; CANCER IN-VITRO; SIRNA DELIVERY; DRUG-DELIVERY; BREAST-CANCER; THERAPY; VIVO; SHELL; COMBINATION; LIPOSOMES;

D O I：

10.1002/anie.201500096

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present a hollow-structured rigid nanovesicle (RNV) fabricated by a multi-stage microfluidic chip in one step, to effectively entrap various hydrophilic reagents inside, without complicated synthesis, extensive use of emulsifiers and stabilizers, and laborious purification procedures. The RNV contains a hollow water core, a rigid poly (lactic-co-glycolic acid) (PLGA) shell, and an outermost lipid layer. The formation mechanism of the RNV is investigated by dissipative particle dynamics (DPD) simulations. The entrapment efficiency of hydrophilic reagents such as calcein, rhodamine B and siRNA inside the hollow water core of RNV is approximate to 90%. In comparison with the combination of free Dox and siRNA, RNV that co-encapsulate siRNA and doxorubicin (Dox) reveals a significantly enhanced anti-tumor effect for a multidrug resistant tumor model.

引用

页码：3952 / 3956

页数：5

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