Evaluation of immune protective efficacies of Eimeria tenella EtMic1 polypeptides with different domain recombination displayed on yeast surface

被引：22

作者：

Chen, Peipei ^{[1
,2
]}

Lv, Junfeng ^{[1
,2
]}

Zhang, Jie ^{[1
,2
]}

Sun, Hui ^{[1
,2
]}

Chen, Zhengtao ^{[1
,2
]}

Li, Hongmei ^{[1
,2
]}

Wang, Fangkun ^{[1
,2
]}

Zhao, Xiaomin ^{[1
,2
]}

机构：

[1] Shandong Agr Univ, Coll Vet Med, Dept Vet Prevent Med, Tai An 271018, Shandong, Peoples R China

[2] Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China

来源：

EXPERIMENTAL PARASITOLOGY | 2015年 / 155卷

基金：

中国国家自然科学基金;

关键词：

EtMic1; Live oral vaccine; TSP domain; Yeast surface display; CEREVISIAE FERMENTATION PRODUCT; SACCHAROMYCES-CEREVISIAE; LIVE VACCINE; PROTEIN; SEQUENCE; ANTIGEN; EXPRESSION; ANTIBODIES; RESPONSES; CHICKENS;

D O I：

10.1016/j.exppara.2015.04.020

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In the present study, three different live oral vaccines using the EBY100/pCTCON-2 yeast surface display system with different Eimeria tenella microneme-1 (EtMic1) protein domain recombination were constructed and their protective efficacies against homologous challenge were compared by evaluating the body weight gains, relative growth rate, cecal lesion scores, oocyst output, oocyst decrease ratio, anticoccidial index, the serum antibody levels and the proliferation ability of blood lymphocytes. The results indicated that all the three constructed live oral vaccines expressing different EtMic1 polypeptides provided excellent protection against homologous challenge by significantly increasing weight gains, reducing cecal lesions, achieving a high ACI, elevating specific antibody response and splenocyte proliferation ability compared with controls. The yeasts displaying the EtMic1 polypeptide-HI (expressed TSP-2, TSP-3 and TSP-4 domains) provided better protection against challenge than the yeasts displaying either the EtMic1 polypeptide-I (expressed I-domain, TSP-1 and TSP-2) or polypeptide-II (expressed I-domain and all the five TSP domains) did. Considering the exclusion of antibiotic resistant gene in the system, the strain EBY100 of Saccaromyces cerevisiae may be a better choice for coccidian antigen delivery. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：1 / 7

页数：7

共 34 条

[1] Antigenicity of infectious pancreatic necrosis virus VP2 subviral particles expressed in yeast [J].